Assessing the detectability of antioxidants in two-dimensional high-performance liquid chromatography

Bassanese, Danielle N., Conlan, Xavier A., Barnett, Neil W. and Stevenson, Paul G. 2015, Assessing the detectability of antioxidants in two-dimensional high-performance liquid chromatography, Journal separation science, vol. 38, no. 10, pp. 1642-1648, doi: 10.1002/jssc.201500054.

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Title Assessing the detectability of antioxidants in two-dimensional high-performance liquid chromatography
Author(s) Bassanese, Danielle N.ORCID iD for Bassanese, Danielle N.
Conlan, Xavier A.ORCID iD for Conlan, Xavier A.
Barnett, Neil W.
Stevenson, Paul G.ORCID iD for Stevenson, Paul G.
Journal name Journal separation science
Volume number 38
Issue number 10
Start page 1642
End page 1648
Total pages 7
Publisher Wiley
Place of publication London, Eng.
Publication date 2015-05
ISSN 1615-9314
Keyword(s) Antioxidants
Limit of detection
Solvent strength mismatch
Two-dimensional high-performance liquid chromatography
Science & Technology
Physical Sciences
Chemistry, Analytical
Summary This paper explores the analytical figures of merit of two-dimensional high-performance liquid chromatography for the separation of antioxidant standards. The cumulative two-dimensional high-performance liquid chromatography peak area was calculated for 11 antioxidants by two different methods--the areas reported by the control software and by fitting the data with a Gaussian model; these methods were evaluated for precision and sensitivity. Both methods demonstrated excellent precision in regards to retention time in the second dimension (%RSD below 1.16%) and cumulative second dimension peak area (%RSD below 3.73% from the instrument software and 5.87% for the Gaussian method). Combining areas reported by the high-performance liquid chromatographic control software displayed superior limits of detection, in the order of 1 × 10(-6) M, almost an order of magnitude lower than the Gaussian method for some analytes. The introduction of the countergradient eliminated the strong solvent mismatch between dimensions, leading to a much improved peak shape and better detection limits for quantification.
Language eng
DOI 10.1002/jssc.201500054
Field of Research 030108 Separation Science
Socio Economic Objective 970103 Expanding Knowledge in the Chemical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID DP140100439
Copyright notice ©2015, Wiley
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