Inquisition of Microcystis aeruginosa and Synechocystis nanowires: characterization and modelling Sure, Sandeep, Torriero, Angel A. J., Gaur, Aditya, Li, Lu Hua, Chen, Ying, Tripathi, Chandrakant, Adholeya, Alok, Ackland, M. Leigh and Kochar, Mandira 2015, Inquisition of Microcystis aeruginosa and Synechocystis nanowires: characterization and modelling, Antonie van Leeuwenhoek: international journal of general and molecular microbiology, vol. 108, no. 5, pp. 1213-1225, doi: 10.1007/s10482-015-0576-2. Attached Files Name Description MIMEType Size Downloads Title Inquisition of Microcystis aeruginosa and Synechocystis nanowires: characterization and modelling Author(s) Sure, Sandeep Torriero, Angel A. J. orcid.org/0000-0001-8616-365X Gaur, Aditya Li, Lu Hua orcid.org/0000-0003-2435-5220 Chen, Ying orcid.org/0000-0002-7322-2224 Tripathi, Chandrakant Adholeya, Alok Ackland, M. Leigh orcid.org/0000-0002-7474-6556 Kochar, Mandira Journal name Antonie van Leeuwenhoek: international journal of general and molecular microbiology Volume number 108 Issue number 5 Start page 1213 End page 1225 Total pages 13 Publisher Springer Place of publication New York, N.Y. Publication date 2015-11 ISSN 1572-9699 Keyword(s) Conductive AFM Microbial nanowires Microcystis aeruginosa Pilus-like structures Synechocystis Summary Identification of extracellular conductive pilus-like structures (PLS) i.e. microbial nanowires has spurred great interest among scientists due to their potential applications in the fields of biogeochemistry, bioelectronics, bioremediation etc. Using conductive atomic force microscopy, we identified microbial nanowires in Microcystis aeruginosa PCC 7806 which is an aerobic, photosynthetic microorganism. We also confirmed the earlier finding that Synechocystis sp. PCC 6803 produces microbial nanowires. In contrast to the use of highly instrumented continuous flow reactors for Synechocystis reported earlier, we identified simple and optimum culture conditions which allow increased production of nanowires in both test cyanobacteria. Production of these nanowires in Synechocystis and Microcystis were found to be sensitive to the availability of carbon source and light intensity. These structures seem to be proteinaceous in nature and their diameter was found to be 4.5-7 and 8.5-11 nm in Synechocystis and M. aeruginosa, respectively. Characterization of Synechocystis nanowires by transmission electron microscopy and biochemical techniques confirmed that they are type IV pili (TFP) while nanowires in M. aeruginosa were found to be similar to an unnamed protein (GenBank : CAO90693.1). Modelling studies of the Synechocystis TFP subunit i.e. PilA1 indicated that strategically placed aromatic amino acids may be involved in electron transfer through these nanowires. This study identifies PLS from Microcystis which can act as nanowires and supports the earlier hypothesis that microbial nanowires are widespread in nature and play diverse roles. Language eng DOI 10.1007/s10482-015-0576-2 Field of Research 060101 Analytical Biochemistry 030102 Electroanalytical Chemistry Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2015, Springer Persistent URL http://hdl.handle.net/10536/DRO/DU:30077859 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Institute for Frontier Materials GTP Research Related Links Link Description Connect to published version Go to link with your DU access privileges   Author URL Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 03 Sep 2015, 07:25:50 EST Fri, 04 Sep 2015, 05:00:15 EST Fri, 04 Sep 2015, 08:16:21 EST Fri, 09 Oct 2015, 17:09:24 EST Mon, 12 Oct 2015, 16:14:55 EST Thu, 12 Nov 2015, 05:15:31 EST Wed, 09 Dec 2015, 11:00:06 EST Wed, 09 Dec 2015, 13:13:29 EST Fri, 21 Apr 2017, 07:13:09 EST Filtered Full Citation counts:   Cited 18 times in TR Web of Science Cited 19 times in Scopus Search Google Scholar Access Statistics: 615 Abstract Views, 4 File Downloads  -  Detailed Statistics Created: Thu, 03 Sep 2015, 07:25:50 EST

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