Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats Maseko, Tebo, Dunshea, Frank Rowland, Howell, Kate, Cho, Hyun-Jung, Rivera, Leni Rose, Furness, John Barton and Ng, Keb 2014, Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats, Nutrients, vol. 6, no. 6, pp. 2478-2492, doi: 10.3390/nu6062478. Attached Files Name Description MIMEType Size Downloads rivera-seleniumenriched-2014.pdf Published version application/pdf 474.25KB 108 Title Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats Formatted title Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats Author(s) Maseko, Tebo Dunshea, Frank Rowland Howell, Kate Cho, Hyun-Jung Rivera, Leni Rose orcid.org/0000-0002-8334-4479 Furness, John Barton Ng, Keb Journal name Nutrients Volume number 6 Issue number 6 Start page 2478 End page 2492 Total pages 15 Publisher MDPI Place of publication Basel, Switzerland Publication date 2014-06-24 ISSN 2072-6643 Keyword(s) Agaricus Animal Feed Animals Gene Expression Regulation, Enzymologic Glutathione Peroxidase Hot Temperature Intestines Male Oxidative Stress Permeability Random Allocation Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Selenium Up-Regulation Se-enriched mushroom Agaricus bisporus heat stress organic Se gut permeability glutathione peroxidise-1 glutathione peroxidise-2 α-tocopherol Summary Dietary effects of organic Se supplementation in the form of Se-enriched Agaricus bisporus mushroom on ileal mucosal permeability and antioxidant selenoenzymes status in heat induced oxidative stress in rats were evaluated. Acute heat stress (40 °C, 21% relative humidity, 90 min exposure) increased ileum baseline short circuit current (Isc; 2.40-fold) and epithelial conductance (Ge; 2.74-fold). Dietary supplementation with Se-enriched A. bisporus (1 µg Se/g feed) reduced (p < 0.05) ileum Isc and Ge during heat stress to 1.74 and 1.91 fold, respectively, indicating protection from heat stress-induced mucosal permeability increase. The expression of ileum glutathione peroxidase (GPx-) 1 and 2 mRNAs were up-regulated (p < 0.05) by 1.90 and 1.87-fold, respectively, for non-heat stress rats on the Se-enriched diet relative to the control. The interplay between heat stress and dietary Se is complex. For rats on the control diet, heat stress alone increased ileum expression of GPx-1 (2.33-fold) and GPx-2 (2.23-fold) relative to thermoneutral conditions. For rats on the Se-enriched diet, heat stress increased (p < 0.05) GPx-1 expression only. Rats on Se-enriched + α-tocopherol diet exhibited increased expression of both genes (p < 0.05). Thus, dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress. Language eng DOI 10.3390/nu6062478 Field of Research 111199 Nutrition and Dietetics not elsewhere classified 1111 Nutrition And Dietetics Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences HERDC Research category C1.1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2014, The Authors Free to Read? 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