The relationship between glial distortion and neuronal changes following intestinal ischemia and reperfusion Thacker, M., Rivera, L.R., Cho, H.-J. and Furness, J.B. 2011, The relationship between glial distortion and neuronal changes following intestinal ischemia and reperfusion, Neurogastroenterology & motility, vol. 23, no. 11, pp. e500-e509, doi: 10.1111/j.1365-2982.2011.01696.x. Attached Files Name Description MIMEType Size Downloads Title The relationship between glial distortion and neuronal changes following intestinal ischemia and reperfusion Author(s) Thacker, M. Rivera, L.R. orcid.org/0000-0002-8334-4479 Cho, H.-J. Furness, J.B. Journal name Neurogastroenterology & motility Volume number 23 Issue number 11 Start page e500 End page e509 Total pages 10 Publisher Wiley-Blackwell Place of publication Chichester, Eng. Publication date 2011-11 ISSN 1350-1925 Keyword(s) enteric glia enteric nervous system glial fibrillary acidic protein Hu ischemia Summary Background Damage to mucosal epithelial cells, musclecells and enteric neurons has been extensivelystudied following intestinal ischemia and reperfusion(I/R). Interestingly, the effects of intestinal I/R on entericglia remains unexplored, despite knowledge thatglia contribute to neuronal maintenance. Here, we describestructural damage to enteric glia and associatedchanges in distribution and immunoreactivity of theneuronal protein Hu. Methods The mouse small intestinewas made ischemic for 3 h and reperfused from 1 to12 h. Immunohistochemical localisation of glial fibrillaryacidic protein (GFAP),HuandTUNELwere used toevaluate changes. Key Results At all time points glialcells became distorted, which was evident by theiraltered GFAP immunoreactivity, including an unusualappearance of bright perinuclear GFAP staining and thepresence of GFAP globules. The numbers of neurons perganglion area were significantly fewer in ganglia thatcontained distorted glia when compared with gangliathat contained glia of normal appearance. The distributionof Hu immunoreactivity was altered at all reperfusiontime points. The presence of vacuoles and Hugranules in neurons was evident and an increase innuclear Hu, relative to cytoplasmic Hu, was observed inganglia that contained both normal and distorted glialcells. A number of neurons appeared to lose their Huimmunoreactivity, most noticeably in ganglia thatcontained distorted glial cells. TUNEL reactionoccurred in a minority of glial cells and neurons.Conclusions & Inferences Structural damage to gliofilamentsoccurs following I/R and may be associatedwith damage to neighboring neurons. Language eng DOI 10.1111/j.1365-2982.2011.01696.x Field of Research 110399 Clinical Sciences not elsewhere classified 1103 Clinical Sciences 1109 Neurosciences Socio Economic Objective 920105 Digestive System Disorders HERDC Research category C1.1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2011, Blackwell Publishing Persistent URL http://hdl.handle.net/10536/DRO/DU:30079271 Document type: Journal Article Collections: Faculty of Health School of Medicine Related Links Link Description Connect to published version Go to link with your DU access privileges   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Tue, 03 Nov 2015, 13:23:02 EST Wed, 04 Nov 2015, 04:01:25 EST Wed, 04 Nov 2015, 08:51:41 EST Tue, 28 Mar 2017, 05:04:25 EST Fri, 28 Apr 2017, 11:19:24 EST Fri, 28 Apr 2017, 11:24:17 EST Sat, 17 Jun 2017, 02:38:23 EST Sat, 25 Nov 2017, 01:32:00 EST Filtered Full Citation counts:   Cited 24 times in TR Web of Science Cited 29 times in Scopus Search Google Scholar Access Statistics: 464 Abstract Views, 2 File Downloads  -  Detailed Statistics Created: Tue, 03 Nov 2015, 13:23:02 EST