Selective enumeration of dairy based strains of probiotic and lactic acid bacteria

Ashraf, Rabia and Smith, Stuart C 2015, Selective enumeration of dairy based strains of probiotic and lactic acid bacteria, International food research journal, vol. 22, no. 6, pp. 2576-2586.

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Title Selective enumeration of dairy based strains of probiotic and lactic acid bacteria
Author(s) Ashraf, Rabia
Smith, Stuart C
Journal name International food research journal
Volume number 22
Issue number 6
Start page 2576
End page 2586
Total pages 11
Publisher Universiti Putra Malaysia
Place of publication Selangor, Malaysia
Publication date 2015-08
ISSN 1985-4668
Keyword(s) probiotic strains
lactic acid bacteria
selective enumeration
Summary Reliable methods for selective enumeration of probiotic and lactic acid bacteria (LAB) are required for improving the functional food quality of probiotics. Various methods were evaluated for selective enumeration of seventeen LAB and probiotic strains. Tested sugars failed to select any species however, improved recovery of total LAB count. The strains were viable and physiologically active within a range of oxygen tension levels, temperature and acidic conditions. Prior methods showed varied results such as De Man Rogosa Sharpe containing bile (MRSB), MRS containing nalidixic acid, paromomycin, neomycin sulphate and lithium chloride (MRS-NPNL), M17 and L. casei (LC) agar failed to select Lactobacillus acidophilus, Bifidobacterium, starter LAB and L. casei strains respectively. However, LC agar appears appropriate for L. paracasei and MRSB for yoghurt starter bacteria in the absence of L. reuteri and L. rhamnosus. The study suggests selective potential of culture media largely depends on target species.
Language eng
Field of Research 0908 Food Sciences
090899 Food Sciences not elsewhere classified
Socio Economic Objective 920411 Nutrition
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, Universiti Putra Malaysia
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Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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