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Chansu inhibits the expression of cortactin in colon cancer cell lines in vitro and in vivo

Li, Chun, Hashimi, Saeed M., Cao, Siyu, Qi, Ji, Good, David, Duan, Wei and Wei, Ming Q. 2015, Chansu inhibits the expression of cortactin in colon cancer cell lines in vitro and in vivo, BMC complementary and alternative medicine, vol. 15, Article Number : 207, pp. 1-8, doi: 10.1186/s12906-015-0723-3.

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Title Chansu inhibits the expression of cortactin in colon cancer cell lines in vitro and in vivo
Formatted title Chansu inhibits the expression of cortactin in colon cancer cell lines in vitro and in vivo
Author(s) Li, Chun
Hashimi, Saeed M.
Cao, Siyu
Qi, Ji
Good, David
Duan, WeiORCID iD for Duan, Wei orcid.org/0000-0001-5782-9184
Wei, Ming Q.
Journal name BMC complementary and alternative medicine
Volume number 15
Season Article Number : 207
Start page 1
End page 8
Total pages 8
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2015
ISSN 1472-6882
Keyword(s) Apoptosis
Chansu
Cinobufagin
Cortactin and xenograft
Summary Background: Chansu is a transitional Chinese medicine that has been used for centuries as therapy for inflammation, anaesthesia and arrhythmia in China and other Asian countries. Recently, it has also been used for anti-cancer purposes. We have previously shown that Chansu has a huge pro-apoptotic potential on colon cancer cells, but to date the detailed mechanism of this action is not well understood.

Methods: One of the major components of Chansu, Cinobufagin (CBF) was used to treat cancer cells. The expressions of levels of cortactin, an important factor in tumour progression and cancer invasion, were assessed in in vitro and in vivo experiments. Additional analyses were performed in subcellular protein fractions and immune-fluorescent staining was used to define cortactin protein expression and the changes of location in CBF-treated cells.

Results:
CBF strongly inhibited the expression of cortactin in HCT116 cells. There were reductions of both mRNA transcription and protein synthesis, which were more significant in the absence of oxygen in vitro. In addition, nuclear translocation of cortactin was observed in HCT116 cells post CBF exposure but not in the negative control, indicating that CBF is likely to interrupt co-localisation of cortactin to cytoskeletal proteins. Most importantly, CBF could diminish the expression of cortactin in human HCT116 xenograft tumours in nude mouse in vivo.

Conclusions: CBF inhibits cortactin expression and nuclear translocation in colon cancer cells in vitro and in mouse models bearing human colon tumour in vivo, suggesting it might disrupt actin-regulated cell movement. Thus, CBF or Chansu could be developed as an effective anti-cancer therapy to stop local invasion and metastasis.
Language eng
DOI 10.1186/s12906-015-0723-3
Field of Research 1104 Complementary And Alternative Medicine
110404 Traditional Chinese Medicine and Treatments
Socio Economic Objective 920102 Cancer and Related Disorders
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2015, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30080599

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.