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A comparison of collection techniques for gene expression analysis of human oral taste tissue

Archer, Nicholas Steven, Liu, Dongli, Shaw, Jan, Hannan, Garry, Duesing, Konsta and Keast, Russell 2016, A comparison of collection techniques for gene expression analysis of human oral taste tissue, PLoS one, vol. 11, no. 3, pp. 1-11, doi: 10.1371/journal.pone.0152157.

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Title A comparison of collection techniques for gene expression analysis of human oral taste tissue
Author(s) Archer, Nicholas Steven
Liu, Dongli
Shaw, Jan
Hannan, Garry
Duesing, Konsta
Keast, RussellORCID iD for Keast, Russell orcid.org/0000-0001-6882-2877
Journal name PLoS one
Volume number 11
Issue number 3
Start page 1
End page 11
Total pages 11
Publisher PLoS
Place of publication San Francisco, Calif.
Publication date 2016
ISSN 1932-6203
Keyword(s) Cheek
Female
Gene Expression Profiling
Humans
Male
Specimen Handling
Taste
Taste Buds
Tongue
Summary Variability in human taste perception is associated with both genetic and environmental factors. The influence of taste receptor expression on this variability is unknown, in part, due to the difficulty in obtaining human oral tissue that enables quantitative expression measures of taste genes. In a comparison of six current techniques (Oragene RNeasy Kit, Isohelix swab, Livibrush cytobrush, tongue saliva, cheek saliva collection, and fungiform papillae biopsy), we identify the fungiform papillae biopsy is the optimal sampling technique to analyse human taste gene expression. The fungiform papillae biopsy resulted in the highest RNA integrity, enabling amplification of all the assessed taste receptor genes (TAS1R1, TAS1R2, TAS1R3, SCNN1A and CD36) and taste tissue marker genes (NCAM1, GNAT3 and PLCβ2). Furthermore, quantitative expression was observed in a subset of taste genes assessed from the saliva collection techniques (cheek saliva, tongue saliva and Oragene RNA kit). These saliva collection techniques may be useful as a non-invasive alternative sampling technique to the fungiform papillae biopsy. Identification of the fungiform papillae biopsy as the optimal collection method will facilitate further research into understanding the effect of gene expression on variability in human taste perception.
Language eng
DOI 10.1371/journal.pone.0152157
Field of Research 090899 Food Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30082607

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.