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Selective enrichment of omega-3 fatty acids in oils by phospholipase A1

Ranjan Moharana, Tushar, Byreddy, Avinesh R., Puri, Munish, Barrow, Colin and Rao, Nalam Madhusudhana 2016, Selective enrichment of omega-3 fatty acids in oils by phospholipase A1, PLoS One, vol. 11, no. 3, Article Number : e0151370, pp. 1-14, doi: 10.1371/journal.pone.0151370.

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Title Selective enrichment of omega-3 fatty acids in oils by phospholipase A1
Author(s) Ranjan Moharana, Tushar
Byreddy, Avinesh R.
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Barrow, ColinORCID iD for Barrow, Colin orcid.org/0000-0002-2153-7267
Rao, Nalam Madhusudhana
Journal name PLoS One
Volume number 11
Issue number 3
Season Article Number : e0151370
Start page 1
End page 14
Total pages 14
Publisher Public Library of Science (PLOS)
Place of publication San Francisco, Calif.
Publication date 2016
ISSN 1932-6203
Summary Omega fatty acids are recognized as key nutrients for healthier ageing. Lipases are used to release ω-3 fatty acids from oils for preparing enriched ω-3 fatty acid supplements. However, use of lipases in enrichment of ω-3 fatty acids is limited due to their insufficient specificity for ω-3 fatty acids. In this study use of phospholipase A1 (PLA1), which possesses both sn-1 specific activity on phospholipids and lipase activity, was explored for hydrolysis of ω-3 fatty acids from anchovy oil. Substrate specificity of PLA1 from Thermomyces lenuginosus was initially tested with synthetic p-nitrophenyl esters along with a lipase from Bacillus subtilis (BSL), as a lipase control. Gas chromatographic characterization of the hydrolysate obtained upon treatment of anchovy oil with these enzymes indicated a selective retention of ω-3 fatty acids in the triglyceride fraction by PLA1 and not by BSL. 13C NMR spectroscopy based position analysis of fatty acids in enzyme treated and untreated samples indicated that PLA1 preferably retained ω-3 fatty acids in oil, while saturated fatty acids were hydrolysed irrespective of their position. Hydrolysis of structured triglyceride,1,3-dioleoyl-2-palmitoylglycerol, suggested that both the enzymes hydrolyse the fatty acids at both the positions. The observed discrimination against ω-3 fatty acids by PLA1 appears to be due to its fatty acid selectivity rather than positional specificity. These studies suggest that PLA1 could be used as a potential enzyme for selective concentrationof ω-3 fatty acids.
Language eng
DOI 10.1371/journal.pone.0151370
Field of Research 100302 Bioprocessing, Bioproduction and Bioproducts
Socio Economic Objective 920411 Nutrition
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30082622

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.