Elite hairy roots of Ocimum basilicum as a new source of rosmarinic acid and antioxidants

Srivastava, Shivani, Conlan, Xavier, Adholeya, Alok and Cahill, David 2016, Elite hairy roots of Ocimum basilicum as a new source of rosmarinic acid and antioxidants, Plant cell, tissue and organ culture, vol. 126, no. 1, pp. 19-32, doi: 10.1007/s11240-016-0973-x.

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Title Elite hairy roots of Ocimum basilicum as a new source of rosmarinic acid and antioxidants
Author(s) Srivastava, Shivani
Conlan, XavierORCID iD for Conlan, Xavier orcid.org/0000-0003-0829-0551
Adholeya, Alok
Cahill, DavidORCID iD for Cahill, David orcid.org/0000-0002-2556-0528
Journal name Plant cell, tissue and organ culture
Volume number 126
Issue number 1
Start page 19
End page 32
Total pages 14
Publisher Springer
Place of publication Berlin, Germany
Publication date 2016-07
ISSN 1573-5044
Keyword(s) Antioxidants
Hairy roots
Ocimum basilicum
Rosmarinic acid
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Plant Sciences
Summary This study reports Agrobacterium rhizogenes-mediated transformation of three cultivars of Ocimum basilicum for hairy root establishment, screening and selection for the production of rosmarinic acid and antioxidants. Hairy root development was found to be explant-specific and virulence-dependent. Distinct inter-cultivar morphological variability was found between the seven axenically developed hairy root lines and morphological traits were found to be correlated with the presence of aux2 genes, their expression and endogenous IAA content. Further inter-cultivar variability in the content of total phenolics, rosmarinic acid and caffeic acid was also found. Production of rosmarinic acid was found to be age-dependent and cultivar-specific. Chemiluminescence analysis showed the hairy roots to be rich in antioxidants and that rosmarinic acid was the major antioxidant molecule. The concentration of rosmarinic acid was found to be positively correlated with the total antioxidant potential of the hairy root extracts. On the basis of origin, morphology and metabolite content, three elite hairy root lines were selected that had significantly higher rosmarinic acid production, biomass and antioxidant potential than non-transformed roots. These new lines are rich reserves of both antioxidants and rosmarinic acid.
Language eng
DOI 10.1007/s11240-016-0973-x
Field of Research 060702 Plant Cell and Molecular Biology
0607 Plant Biology
0703 Crop And Pasture Production
Socio Economic Objective 970106 Expanding Knowledge in the Biological Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, Springer Science+Business Media
Persistent URL http://hdl.handle.net/10536/DRO/DU:30083359

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