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Zebrafish embryonic lipidomic analysis reveals that the yolk cell as metabolically active in processing lipid

Fraher, Daniel, Sanigorski, Andrew, Mellett, Natalie A., Meikle, Peter J., Sinclair, Andrew J. and Gibert, Yann 2016, Zebrafish embryonic lipidomic analysis reveals that the yolk cell as metabolically active in processing lipid, Cell reports, vol. 14, no. 6, pp. 1317-1329, doi: 10.1016/j.celrep.2016.01.016.

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Title Zebrafish embryonic lipidomic analysis reveals that the yolk cell as metabolically active in processing lipid
Author(s) Fraher, Daniel
Sanigorski, AndrewORCID iD for Sanigorski, Andrew orcid.org/0000-0002-2858-4621
Mellett, Natalie A.
Meikle, Peter J.
Sinclair, Andrew J.
Gibert, Yann
Journal name Cell reports
Volume number 14
Issue number 6
Start page 1317
End page 1329
Total pages 14
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2016-02-16
ISSN 2211-1247
Keyword(s) BODIPY
PPARγ
TLC
embryo body
embryogenesis
lipidomics
lipids
yolk sac
zebrafish
Summary The role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition and examined expression of key lipid-regulating genes during zebrafish embryogenesis. We also conducted a semiquantitative analysis of lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, we analyzed processing of boron-dipyrromethene (BODIPY) lipid analogs injected into the yolk using thin layer chromatography. Our data reveal that the most abundant lipids in the embryo are cholesterol, phosphatidylcholine, and triglyceride. Moreover, we demonstrate that lipids are processed within the yolk prior to mobilization to the embryonic body. Our data identify a metabolically active yolk and body resulting in a dynamic lipid composition. This provides a foundation for studying lipid biology during normal or pharmacologically compromised embryogenesis.
Language eng
DOI 10.1016/j.celrep.2016.01.016
Field of Research 119999 Medical and Health Sciences not elsewhere classified
Socio Economic Objective 929999 Health not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution Non-Commercial No-Derivatives licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30085216

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.