Zebrafish embryonic lipidomic analysis reveals that the yolk cell as metabolically active in processing lipid Fraher, Daniel, Sanigorski, Andrew, Mellett, Natalie A., Meikle, Peter J., Sinclair, Andrew J. and Gibert, Yann 2016, Zebrafish embryonic lipidomic analysis reveals that the yolk cell as metabolically active in processing lipid, Cell reports, vol. 14, no. 6, pp. 1317-1329, doi: 10.1016/j.celrep.2016.01.016. Attached Files Name Description MIMEType Size Downloads fraher-zebrafishembryonic-2016.pdf Published version application/pdf 3.66MB 110 Title Zebrafish embryonic lipidomic analysis reveals that the yolk cell as metabolically active in processing lipid Author(s) Fraher, Daniel Sanigorski, Andrew orcid.org/0000-0002-2858-4621 Mellett, Natalie A. Meikle, Peter J. Sinclair, Andrew J. Gibert, Yann Journal name Cell reports Volume number 14 Issue number 6 Start page 1317 End page 1329 Total pages 14 Publisher Elsevier Place of publication Amsterdam, The Netherlands Publication date 2016-02-16 ISSN 2211-1247 Keyword(s) BODIPY PPARγ TLC embryo body embryogenesis lipidomics lipids yolk sac zebrafish Summary The role of lipids in providing energy and structural cellular components during vertebrate development is poorly understood. To elucidate these roles further, we visualized lipid deposition and examined expression of key lipid-regulating genes during zebrafish embryogenesis. We also conducted a semiquantitative analysis of lipidomic composition using liquid chromatography (LC)-mass spectrometry. Finally, we analyzed processing of boron-dipyrromethene (BODIPY) lipid analogs injected into the yolk using thin layer chromatography. Our data reveal that the most abundant lipids in the embryo are cholesterol, phosphatidylcholine, and triglyceride. Moreover, we demonstrate that lipids are processed within the yolk prior to mobilization to the embryonic body. Our data identify a metabolically active yolk and body resulting in a dynamic lipid composition. This provides a foundation for studying lipid biology during normal or pharmacologically compromised embryogenesis. Language eng DOI 10.1016/j.celrep.2016.01.016 Field of Research 119999 Medical and Health Sciences not elsewhere classified Socio Economic Objective 929999 Health not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2016, The Authors Free to Read? Yes Use Rights Persistent URL http://hdl.handle.net/10536/DRO/DU:30085216 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to published version Go to link with your DU access privileges   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Wed, 03 Aug 2016, 12:19:29 EST Thu, 04 Aug 2016, 05:01:53 EST Sat, 06 Aug 2016, 06:09:27 EST Mon, 10 Oct 2016, 09:15:10 EST Mon, 10 Oct 2016, 09:17:28 EST Mon, 10 Oct 2016, 09:24:27 EST Fri, 07 Sep 2018, 12:11:43 EST Filtered Full Citation counts:   Cited 114 times in TR Web of Science Cited 115 times in Scopus Search Google Scholar Access Statistics: 542 Abstract Views, 111 File Downloads  -  Detailed Statistics Created: Wed, 03 Aug 2016, 12:19:29 EST