Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid Markworth, James F., Kaur, Gunveen, Miller, Eliza G., Larsen, Amy E., Sinclair, Andrew J., Maddipati, Krishna Rao and Cameron-Smith, David 2016, Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid, FASEB journal, vol. 30, no. 11, pp. 3714-3725, doi: 10.1096/fj.201600360R. Attached Files Name Description MIMEType Size Downloads Title Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid Author(s) Markworth, James F. Kaur, Gunveen orcid.org/0000-0002-6250-0495 Miller, Eliza G. Larsen, Amy E. Sinclair, Andrew J. Maddipati, Krishna Rao Cameron-Smith, David Journal name FASEB journal Volume number 30 Issue number 11 Start page 3714 End page 3725 Total pages 17 Publisher Federation of American Societies for Experimental Biology Place of publication Bethesda, M.D. Publication date 2016-11 ISSN 0892-6638 1530-6860 Keyword(s) eicosanoids fish oil inflammation lipidomics omega-3 fatty acids Summary In contrast to the well-characterized effects of specialized proresolving lipid mediators (SPMs) derived from eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), little is known about the metabolic fate of the intermediary long-chain (LC) n-3 polyunsaturated fatty acid (PUFA) docosapentaenoic acid (DPA). In this double blind crossover study, shifts in circulating levels of n-3 and n-6 PUFA-derived bioactive lipid mediators were quantified by an unbiased liquid chromatography-tandem mass spectrometry lipidomic approach. Plasma was obtained from human subjects before and after 7 d of supplementation with pure n-3 DPA, n-3 EPA or placebo (olive oil). DPA supplementation increased the SPM resolvin D5n-3DPA (RvD5n-3DPA) and maresin (MaR)-1, the DHA vicinal diol 19,20-dihydroxy-DPA and n-6 PUFA derived 15-keto-PG E2 (15-keto-PGE2). EPA supplementation had no effect on any plasma DPA or DHA derived mediators, but markedly elevated monohydroxy-eicosapentaenoic acids (HEPEs), including the e-series resolvin (RvE) precursor 18-HEPE; effects not observed with DPA supplementation. These data show that dietary n-3 DPA and EPA have highly divergent effects on human lipid mediator profile, with no overlap in PUFA metabolites formed. The recently uncovered biologic activity of n-3 DPA docosanoids and their marked modulation by dietary DPA intake reveals a unique and specific role of n-3 DPA in human physiology.-Markworth, J. F., Kaur, G., Miller, E. G., Larsen, A. E., Sinclair, A. J., Maddipati, K. R., Cameron-Smith, D. Divergent shifts in lipid mediator profile following supplementation with n-3 docosapentaenoic acid and eicosapentaenoic acid. Language eng DOI 10.1096/fj.201600360R Field of Research 0601 Biochemistry And Cell Biology 0606 Physiology 1116 Medical Physiology Socio Economic Objective 929999 Health not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2016, Federation of American Societies for Experimental Biology Persistent URL http://hdl.handle.net/10536/DRO/DU:30085621 Document type: Journal Article Collections: Faculty of Health School of Exercise and Nutrition Sciences School of Medicine Institute for Physical Activity and Nutrition Related Links Link Description Connect to Elements publication management system Go to link with your DU access privileges   Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Tue, 23 Aug 2016, 14:02:30 EST Wed, 24 Aug 2016, 05:00:31 EST Wed, 24 Aug 2016, 06:12:00 EST Wed, 02 Nov 2016, 13:27:30 EST Wed, 02 Nov 2016, 13:32:49 EST Wed, 02 Nov 2016, 13:33:49 EST Wed, 11 Jan 2017, 09:47:00 EST Wed, 11 Jan 2017, 09:59:44 EST Mon, 12 Mar 2018, 12:53:43 EST Mon, 11 Nov 2019, 07:41:14 EST Thu, 25 Feb 2021, 11:35:30 EST Filtered Full Citation counts:   Cited 55 times in TR Web of Science Cited 55 times in Scopus Search Google Scholar Access Statistics: 588 Abstract Views, 4 File Downloads  -  Detailed Statistics Created: Tue, 23 Aug 2016, 14:02:30 EST