Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase)

McCleary, Barry V., Mangan, David, Daly, Robin, Fort, Sebastien, Ivory, Ruth and McCormack, Niall 2014, Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase), Carbohydrate research, vol. 385, pp. 9-17, doi: 10.1016/j.carres.2013.12.001.

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Title Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase)
Formatted title Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase)
Author(s) McCleary, Barry V.
Mangan, David
Daly, RobinORCID iD for Daly, Robin
Fort, Sebastien
Ivory, Ruth
McCormack, Niall
Journal name Carbohydrate research
Volume number 385
Start page 9
End page 17
Total pages 9
Publisher Elsevier
Place of publication Amsterdam, The Netherlands
Publication date 2014-02-19
ISSN 0008-6215
Keyword(s) endo-1,4-β-glucanase
assay procedure
Summary A specific and sensitive substrate for the assay of endo-1,4-β-glucanase (cellulase) has been prepared. The substrate mixture comprises benzylidene end-blocked 2-chloro-4-nitrophenyl-β-cellotrioside (BzCNPG3) in the presence of thermostable β-glucosidase. Hydrolysis by exo-acting enzymes such as β-glucosidase and exo-β-glucanase is prevented by the presence of the benzylidene group on the non-reducing end ᴅ-glucosyl residue. On hydrolysis by cellulase, the 2-chloro-4-nitrophenyl-β-glycoside is immediately hydrolysed to 2-chloro-4-nitrophenol and free ᴅ-glucose by the β-glucosidase in the substrate mixture. The reaction is terminated and colour developed by the addition of a weak alkaline solution. The assay procedure is simple to use, specific, accurate, robust and readily adapted to automation. This procedure should find widespread applications in biomass enzymology and in the specific assay of endo-1,4-β-glucanase in general.
Language eng
DOI 10.1016/j.carres.2013.12.001
Field of Research 060199 Biochemistry and Cell Biology not elsewhere classified
0305 Organic Chemistry
0601 Biochemistry And Cell Biology
0304 Medicinal And Biomolecular Chemistry
Socio Economic Objective 970111 Expanding Knowledge in the Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2013, Elsevier
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Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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