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Metal ion type significantly affects the morphology but not the activity of lipase-metal-phosphate nanoflowers

Sharma, N, Parhizkar, M, Cong, W, Mateti, S, Kirkland, MA, Puri, M and Sutti, A 2017, Metal ion type significantly affects the morphology but not the activity of lipase-metal-phosphate nanoflowers, RSC advances, vol. 7, no. 41, pp. 25437-25443, doi: 10.1039/c7ra00302a.

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Title Metal ion type significantly affects the morphology but not the activity of lipase-metal-phosphate nanoflowers
Author(s) Sharma, N
Parhizkar, MORCID iD for Parhizkar, M orcid.org/0000-0001-9462-7901
Cong, W
Mateti, SORCID iD for Mateti, S orcid.org/0000-0002-3888-6420
Kirkland, MA
Puri, MORCID iD for Puri, M orcid.org/0000-0003-2469-3326
Sutti, AORCID iD for Sutti, A orcid.org/0000-0002-1793-3881
Journal name RSC advances
Volume number 7
Issue number 41
Start page 25437
End page 25443
Total pages 7
Publisher Royal Society of Chemistry
Place of publication Cambridge, Eng.
Publication date 2017
ISSN 2046-2069
Keyword(s) Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
Inorganic hybrid nanoflowers
Enzyme immobilization
Nanomaterials
Biocatalysts
Biodiesel
Summary Enzyme–metal-ion–phosphate nanoflowers are high-surface area materials which are known to show higher activity than the constituting protein. Although the synthesis of hybrid nanoflowers has been demonstrated with a variety of proteins and reaction conditions, only di-valent metal ions have been tested to date. We expand on previous findings by testing a range of metal ions of different valence in co-presence with lipase from Burkholderia cepacia: Ag(I), Fe(II), Cu(II), Au(III). All metal ions produced colour precipitates, although the type of metal caused different precipitate morphologies under comparable reaction conditions: from nanoflowers to forests of nano-plates and crystal-like precipitates. In contrast, the type of metal ion did not appear to significantly affect the product's specific enzyme activity, which remained greater than that of free lipase. This indicates that the type of metal ion and the macroscopic arrangement of the petals play a secondary role to that of the co-presence of the metal and phosphate ions in determining lipase nanoflower activity. The demonstrated ability to produce metal–phosphate-protein nanoflowers with a selection of different metals also opens the way to producing a wider range of functional, nanostructured, materials.
Language eng
DOI 10.1039/c7ra00302a
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30097162

Document type: Journal Article
Collections: Institute for Frontier Materials
Open Access Collection
GTP Research
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.