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Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells

Al Shamaileh, Hadi, Wang, Tao, Xiang, Dongxi, Yin, Wang, Tran, Ha Lien Phuong, Barrero, Roberto A., Zhang, Pei-Zhuo, Li, Yong, Kong, Lingxue, Liu, Ke, Zhou, Shu-Feng, Hou, Yingchun, Shigdar, Sarah and Duan, Wei 2017, Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells, Scientific reports, vol. 7, no. 1, pp. 1-9, doi: 10.1038/s41598-017-05859-z.

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Title Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
Author(s) Al Shamaileh, Hadi
Wang, Tao
Xiang, Dongxi
Yin, WangORCID iD for Yin, Wang orcid.org/0000-0002-5665-3559
Tran, Ha Lien PhuongORCID iD for Tran, Ha Lien Phuong orcid.org/0000-0001-8463-7516
Barrero, Roberto A.
Zhang, Pei-Zhuo
Li, Yong
Kong, LingxueORCID iD for Kong, Lingxue orcid.org/0000-0001-6219-3897
Liu, Ke
Zhou, Shu-Feng
Hou, Yingchun
Shigdar, SarahORCID iD for Shigdar, Sarah orcid.org/0000-0001-8084-8447
Duan, WeiORCID iD for Duan, Wei orcid.org/0000-0001-5782-9184
Journal name Scientific reports
Volume number 7
Issue number 1
Article ID 5898
Start page 1
End page 9
Total pages 9
Publisher Nature Publishing Group
Place of publication London, Eng.
Publication date 2017-07-19
ISSN 2045-2322
Keyword(s) Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
Summary The development of chemoresistance and inability in elimination of cancer stem cells are among the key limitations of cancer chemotherapy. Novel molecular therapeutic strategies able to overcome such limitations are urgently needed for future effective management of cancer. In this report, we show that EpCAM-aptamer-guided survivin RNAi effectively downregulated survivin both in colorectal cancer cells in vitro and in a mouse xenograft model for colorectal cancer. When combined with the conventional chemotherapeutic agents, the aptamer-guided survivin RNAi was able to enhance the sensitivity towards 5-FU or oxaliplatin in colorectal cancer stem cells, increase apoptosis, inhibit tumour growth and improve the overall survival of mice bearing xenograft colorectal cancer. Our results indicate that survivin is one of the key players responsible for the innate chemoresistance of colorectal cancer stem cells. Thus, aptamer-mediated targeting of survivin in cancer stem cells in combination with chemotherapeutic drugs constitutes a new avenue to improve treatment outcome in oncologic clinics.
Language eng
DOI 10.1038/s41598-017-05859-z
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2017, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30099172

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Created: Mon, 24 Jul 2017, 16:16:14 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.