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Analysis of Anoxybacillus genomes from the aspects of lifestyle adaptations, prophage diversity, and carbohydrate metabolism

Goh, Kian Mau, Gan, Han Ming, Chan, Kok-Gan, Chan, Giek Far, Shahar, Saleha, Chong, Chun Shiong, Kahar, Ummirul Mukminin and Chai, Kian Piaw 2014, Analysis of Anoxybacillus genomes from the aspects of lifestyle adaptations, prophage diversity, and carbohydrate metabolism, PLoS one, vol. 9, no. 3, pp. 1-15, doi: 10.1371/journal.pone.0090549.

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Title Analysis of Anoxybacillus genomes from the aspects of lifestyle adaptations, prophage diversity, and carbohydrate metabolism
Author(s) Goh, Kian Mau
Gan, Han MingORCID iD for Gan, Han Ming orcid.org/0000-0001-7987-738X
Chan, Kok-Gan
Chan, Giek Far
Shahar, Saleha
Chong, Chun Shiong
Kahar, Ummirul Mukminin
Chai, Kian Piaw
Journal name PLoS one
Volume number 9
Issue number 3
Article ID e90549
Start page 1
End page 15
Total pages 15
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2014-03
ISSN 1932-6203
Keyword(s) adaptation, physiological
Anoxybacillus
bacterial proteins
carbohydrate metabolism
DNA repair
DNA, bacterial
gene transfer, horizontal
genetic variation
genome, bacterial
genomic instability
glycoside hydrolases
hot springs
molecular sequence annotation
multigene family
phylogeny
prophages
sequence analysis, DNA
viral proteins
water microbiology
Summary Species of Anoxybacillus are widespread in geothermal springs, manure, and milk-processing plants. The genus is composed of 22 species and two subspecies, but the relationship between its lifestyle and genome is little understood. In this study, two high-quality draft genomes were generated from Anoxybacillus spp. SK3-4 and DT3-1, isolated from Malaysian hot springs. De novo assembly and annotation were performed, followed by comparative genome analysis with the complete genome of Anoxybacillus flavithermus WK1 and two additional draft genomes, of A. flavithermus TNO-09.006 and A. kamchatkensis G10. The genomes of Anoxybacillus spp. are among the smaller of the family Bacillaceae. Despite having smaller genomes, their essential genes related to lifestyle adaptations at elevated temperature, extreme pH, and protection against ultraviolet are complete. Due to the presence of various competence proteins, Anoxybacillus spp. SK3-4 and DT3-1 are able to take up foreign DNA fragments, and some of these transferred genes are important for the survival of the cells. The analysis of intact putative prophage genomes shows that they are highly diversified. Based on the genome analysis using SEED, many of the annotated sequences are involved in carbohydrate metabolism. The presence of glycosyl hydrolases among the Anoxybacillus spp. was compared, and the potential applications of these unexplored enzymes are suggested here. This is the first study that compares Anoxybacillus genomes from the aspect of lifestyle adaptations, the capacity for horizontal gene transfer, and carbohydrate metabolism.
Language eng
DOI 10.1371/journal.pone.0090549
Field of Research 060408 Genomics
060503 Microbial Genetics
060501 Bacteriology
MD Multidisciplinary
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2014, Goh et al
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30101920

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.