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Helicobacter pylori eradication causes perturbation of the human gut microbiome in young adults

Yap, Theresa Wan-Chen, Gan, Han Ming, Lee, Yin-Peng, Leow, Alex Hwong-Ruey, Azmi, Ahmad Najib, Francois, Fritz, Perez-Perez, Guillermo I., Loke, Mun-Fai, Goh, Khean-Lee and Vadivelu, Jamuna 2016, Helicobacter pylori eradication causes perturbation of the human gut microbiome in young adults, PLoS one, vol. 11, no. 3, pp. 1-20, doi: 10.1371/journal.pone.0151893.

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Title Helicobacter pylori eradication causes perturbation of the human gut microbiome in young adults
Author(s) Yap, Theresa Wan-Chen
Gan, Han MingORCID iD for Gan, Han Ming orcid.org/0000-0001-7987-738X
Lee, Yin-Peng
Leow, Alex Hwong-Ruey
Azmi, Ahmad Najib
Francois, Fritz
Perez-Perez, Guillermo I.
Loke, Mun-Fai
Goh, Khean-Lee
Vadivelu, Jamuna
Journal name PLoS one
Volume number 11
Issue number 3
Article ID e0151893
Start page 1
End page 20
Total pages 20
Publisher PLoS
Place of publication San Francisco, Calif.
Publication date 2016
ISSN 1932-6203
Keyword(s) Adolescent
Adult
Anti-Bacterial Agents
Cohort Studies
Feces
Female
Gastrointestinal Microbiome
Helicobacter Infections
Humans
Male
Metagenomics
Young Adult
Summary BACKGROUND: Accumulating evidence shows that Helicobacter pylori protects against some metabolic and immunological diseases in which the development of these diseases coincide with temporal or permanent dysbiosis. The aim of this study was to assess the effect of H. pylori eradication on the human gut microbiome.

METHODS: As part of the currently on-going ESSAY (Eradication Study in Stable Adults/Youths) study, we collected stool samples from 17 H. pylori-positive young adult (18-30 years-old) volunteers. The same cohort was followed up 6, 12 and 18 months-post H. pylori eradication. The impact of H. pylori on the human gut microbiome pre- and post-eradication was investigated using high throughput 16S rRNA gene (V3-V4 region) sequencing using the Illumina Miseq followed by data analysis using Qiime pipeline.

RESULTS: We compared the composition and diversity of bacterial communities in the fecal microbiome of the H. pylori-positive volunteers, before and after H. pylori eradication therapy. The 16S rRNA gene was sequenced at an average of 150,000-170,000 reads/sample. The microbial diversity were similar pre- and post-H. pylori eradication with no significant differences in richness and evenness of bacterial species. Despite that the general profile of the gut microbiome was similar pre- and post-eradication, some changes in the bacterial communities at the phylum and genus levels were notable, particularly the decrease in relative abundance of Bacterioidetes and corresponding increase in Firmicutes after H. pylori eradication. The significant increase of short-chain fatty acids (SCFA)-producing bacteria genera could also be associated with increased risk of metabolic disorders.

CONCLUSIONS: Our preliminary stool metagenomics study shows that eradication of H. pylori caused perturbation of the gut microbiome and may indirectly affect the health of human. Clinicians should be aware of the effect of broad spectrum antibiotics used in H. pylori eradication regimen and be cautious in the clinical management of H. pylori infection, particularly in immunocompromised patients.
Language eng
DOI 10.1371/journal.pone.0151893
Field of Research 060408 Genomics
060503 Microbial Genetics
MD Multidisciplinary
HERDC Research category C1.1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Copyright notice ©2016, The Authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30101937

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.