The ADAMTS5 metzincin regulates zebrafish somite differentiation Dancevic, Carolyn M, Gibert, Yann, Berger, Joachim, Smith, Adam D, Liongue, Clifford, Stupka, Nicole, Ward, Alister C and McCulloch, Daniel R 2018, The ADAMTS5 metzincin regulates zebrafish somite differentiation, International journal of molecular sciences, vol. 19, no. 3, pp. 1-12, doi: 10.3390/ijms19030766. Attached Files Name Description MIMEType Size Downloads ward-adamts5metzincin-2018.pdf Published version application/pdf 6.29MB 100 Title The ADAMTS5 metzincin regulates zebrafish somite differentiation Author(s) Dancevic, Carolyn M Gibert, Yann Berger, Joachim Smith, Adam D Liongue, Clifford orcid.org/0000-0002-1646-4905 Stupka, Nicole orcid.org/0000-0002-1000-1707 Ward, Alister C orcid.org/0000-0001-7945-7975 McCulloch, Daniel R Journal name International journal of molecular sciences Volume number 19 Issue number 3 Article ID 766 Start page 1 End page 12 Total pages 12 Publisher MDPI Place of publication Basel, Switzerland Publication date 2018-03-07 ISSN 1422-0067 1422-0067 Keyword(s) metalloproteinase extracellular matrix ADAMTS somite muscle zebrafish science & technology life sciences & biomedicine physical sciences biochemistry & molecular biology chemistry Summary The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of myod expression by ADAMTS5 and Shh during somite differentiation was observed. These roles were not dependent upon the catalytic activity of ADAMTS5. These data identify a non-enzymatic function for ADAMTS5 in regulating an important cell signaling pathway that impacts on muscle development, with implications for musculoskeletal diseases in which ADAMTS5 and Shh have been associated. Language eng DOI 10.3390/ijms19030766 Field of Research 0399 Other Chemical Sciences 0604 Genetics 0699 Other Biological Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2018, the authors Free to Read? Yes Use Rights Persistent URL http://hdl.handle.net/10536/DRO/DU:30110235 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to Elements publication management system Go to link with your DU access privileges   Connect to published version (open access) Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Mon, 09 Jul 2018, 11:49:58 EST Mon, 09 Jul 2018, 13:43:20 EST Mon, 09 Jul 2018, 13:57:30 EST Fri, 27 Jul 2018, 09:16:31 EST Tue, 31 Jul 2018, 15:01:09 EST Tue, 31 Jul 2018, 15:03:24 EST Tue, 31 Jul 2018, 15:04:07 EST Wed, 01 Aug 2018, 11:26:23 EST Fri, 07 Sep 2018, 18:55:58 EST Filtered Full Citation counts:   Cited 2 times in TR Web of Science Cited 2 times in Scopus Search Google Scholar Access Statistics: 468 Abstract Views, 101 File Downloads  -  Detailed Statistics Created: Mon, 09 Jul 2018, 11:49:58 EST