Manufacturable system for zoonotic disease detection Doeven, Egan, Nai, Yi Heng, Alexander, Richard, Haswell, S and Guijt, Rosanne 2018, Manufacturable system for zoonotic disease detection, in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Curran Associates,, pp. 2437-2438. Attached Files Name Description MIMEType Size Downloads Title Manufacturable system for zoonotic disease detection Author(s) Doeven, Egan orcid.org/0000-0003-2677-4269 Nai, Yi Heng orcid.org/0000-0003-4998-7574 Alexander, Richard orcid.org/0000-0001-6121-7885 Haswell, S Guijt, Rosanne orcid.org/0000-0003-0011-5708 Conference name MicroTAS 2018 Conference location Kaohsiung, Taiwan Conference dates 2018/11/11 - 2018/11/18 Title of proceedings 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 Publication date 2018 Start page 2437 End page 2438 Total pages 2 Publisher Curran Associates Summary Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. A manufacturable microfluidic system containing an injection-molded device for sample in/answer out analysis of a buccal swab lysate for avian influenza detection is presented. Fluidic processing was done using an external magnet to move paramagnetic particles, and the optimized injection moulding process allowed for side illuminated fluorescence detection with minimal hardware. The optimised amplification chemistry allowed for one-pot, truly isothermal Nucleic Acid Sequence Based Amplification. The chip design allows for simultaneous extraction, amplification and analysis of 5 targets. The system allows for the detection of 5000 copies of viral RNA (∼1.106copies/mL) using on-chip extraction of a 30 μL sample; 1.104 copies/mL with off-chip extraction. ISBN 9781510897571 Indigenous content off HERDC Research category E3 Extract of paper Persistent URL http://hdl.handle.net/10536/DRO/DU:30135538 Document type: Conference Paper Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences   Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Tue, 10 Mar 2020, 09:24:00 EST Wed, 11 Mar 2020, 04:00:40 EST Wed, 11 Mar 2020, 05:04:18 EST Sun, 07 Nov 2021, 12:37:02 EST Sun, 07 Nov 2021, 13:19:41 EST Sun, 07 Nov 2021, 13:34:42 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 12 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Tue, 10 Mar 2020, 09:24:00 EST