Low-cost blood plasma separation method using salt functionalized paper

Nilghaz, Azadeh and Shen, Wei 2015, Low-cost blood plasma separation method using salt functionalized paper, RSC Advances, vol. 5, no. 66, pp. 53172-53179, doi: 10.1039/c5ra01468a.

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Title Low-cost blood plasma separation method using salt functionalized paper
Author(s) Nilghaz, AzadehORCID iD for Nilghaz, Azadeh orcid.org/0000-0001-5769-7281
Shen, Wei
Journal name RSC Advances
Volume number 5
Issue number 66
Start page 53172
End page 53179
Total pages 8
Publisher Royal Society of Chemistry
Place of publication Cambridge, Eng.
Publication date 2015-06-08
ISSN 2046-2069
Keyword(s) Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Summary This study describes an extremely low-cost method for separating plasma in a sample of whole human blood on salt functionalized paper by means of osmotic pressure. When a sample of whole blood was introduced onto the salt functionalized paper, plasma dissolves the salt and places the red blood cells (RBCs) in a hypertonic medium. This leads to the generation of osmotic pressure across the cells membrane, and also the crenation of RBCs. The effect of different concentrations of salt on RBC deformation and crenation has been monitored using confocal microscopy. Depending upon the salt concentration, RBCs deform into various shapes under osmotic pressure. At high salt concentration, RBCs turn into deflated thin disks. This increases the RBCs' contact with one another and with fibres in the paper as well. Besides, the counter ion valency charge of the Na+ suppresses the thickness of the charged double layer of RBC. Subsequently, aggregation of the deflated RBCs occurs. The aggregates are large enough to be separated chromatographically from the plasma phase of the wicking front. Our results show that 0.5 μL addition of 0.68 M (4% w/v) saline solution (NaCl) can provide sufficient plasma separation on a filter paper for diagnostic applications. A colorimetric blood glucose concentration assay is employed to demonstrate the efficiency of this plasma separation method on paper. The experimental investigation indicates that although the crenation of RBCs forced a small amount of water into plasma, this method is suitable for performing glucose assay in human blood on paper. Our method can enhance bioassays performed on microfluidic paper-based analytical devices (μPADs) by combining the separation and testing of plasma into a single device with no significant additional cost.
Language eng
DOI 10.1039/c5ra01468a
Indigenous content off
Field of Research 03 Chemical Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2015, Royal Society of Chemistry
Persistent URL http://hdl.handle.net/10536/DRO/DU:30138838

Document type: Journal Article
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