MicroRNA-99b-5p downregulates protein synthesis in human primary myotubes

Zacharewicz, E, Kalanon, M, Murphy, RM, Russell, Aaron and Lamon, Severine 2020, MicroRNA-99b-5p downregulates protein synthesis in human primary myotubes, American journal of physiology. Cell physiology, vol. 319, no. 2, pp. C432-C440, doi: 10.1152/ajpcell.00172.2020.

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Title MicroRNA-99b-5p downregulates protein synthesis in human primary myotubes
Author(s) Zacharewicz, E
Kalanon, M
Murphy, RM
Russell, AaronORCID iD for Russell, Aaron orcid.org/0000-0002-7323-9501
Lamon, SeverineORCID iD for Lamon, Severine orcid.org/0000-0002-3271-6551
Journal name American journal of physiology. Cell physiology
Volume number 319
Issue number 2
Start page C432
End page C440
Total pages 9
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2020-08
ISSN 0363-6143
Keyword(s) microRNA
primary muscle cells
protein synthesis
Science & Technology
Life Sciences & Biomedicine
Cell Biology
Summary microRNAs (miRNAs) are important regulators of cellular homeostasis and exert their effect by directly controlling protein expression. We have previously reported an age-dependent negative association between microRNA-99b (miR-99b-5p) expression and muscle protein synthesis in human muscle in vivo. Here we investigated the role of miR-99b-5p as a potential negative regulator of protein synthesis via inhibition of mammalian target for rapamycin (MTOR) signaling in human primary myocytes. Overexpressing miR-99b-5p in human primary myotubes from young and old subjects significantly decreased protein synthesis with no effect of donor age. A binding interaction between miR-99b-5p and its putative binding site within the MTOR 3′-untranslated region (UTR) was confirmed in C2C12 myoblasts. The observed decline in protein synthesis was, however, not associated with a suppression of the MTOR protein but of its regulatory associated protein of mTOR complex 1 (RPTOR). These results demonstrate that modulating the expression levels of a miRNA can regulate protein synthesis in human muscle cells and provide a potential mechanism for muscle wasting in vivo.
Language eng
DOI 10.1152/ajpcell.00172.2020
Indigenous content off
Field of Research 0601 Biochemistry and Cell Biology
0606 Physiology
1116 Medical Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30139860

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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