MicroRNA-99b-5p downregulates protein synthesis in human primary myotubes

Zacharewicz, E, Kalanon, M, Murphy, RM, Russell, Aaron and Lamon, Severine 2020, MicroRNA-99b-5p downregulates protein synthesis in human primary myotubes, American journal of physiology. Cell physiology, vol. 319, no. 2, pp. C432-C440, doi: 10.1152/ajpcell.00172.2020.

Attached Files
Name Description MIMEType Size Downloads

Title MicroRNA-99b-5p downregulates protein synthesis in human primary myotubes
Author(s) Zacharewicz, E
Kalanon, M
Murphy, RM
Russell, AaronORCID iD for Russell, Aaron orcid.org/0000-0002-7323-9501
Lamon, SeverineORCID iD for Lamon, Severine orcid.org/0000-0002-3271-6551
Journal name American journal of physiology. Cell physiology
Volume number 319
Issue number 2
Start page C432
End page C440
Total pages 9
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2020-08
ISSN 0363-6143
1522-1563
Keyword(s) microRNA
muscle
primary muscle cells
protein synthesis
Science & Technology
Life Sciences & Biomedicine
Cell Biology
Physiology
SKELETAL-MUSCLE HYPERTROPHY
GROWTH-FACTOR-I
RESISTANCE EXERCISE
PHOSPHATIDYLINOSITOL 3-KINASE
MAMMALIAN TARGET
CELL-CYCLE
EXPRESSION
INSULIN
YOUNG
ACTIVATION
Summary microRNAs (miRNAs) are important regulators of cellular homeostasis and exert their effect by directly controlling protein expression. We have previously reported an age-dependent negative association between microRNA-99b (miR-99b-5p) expression and muscle protein synthesis in human muscle in vivo. Here we investigated the role of miR-99b-5p as a potential negative regulator of protein synthesis via inhibition of mammalian target for rapamycin (MTOR) signaling in human primary myocytes. Overexpressing miR-99b-5p in human primary myotubes from young and old subjects significantly decreased protein synthesis with no effect of donor age. A binding interaction between miR-99b-5p and its putative binding site within the MTOR 3′-untranslated region (UTR) was confirmed in C2C12 myoblasts. The observed decline in protein synthesis was, however, not associated with a suppression of the MTOR protein but of its regulatory associated protein of mTOR complex 1 (RPTOR). These results demonstrate that modulating the expression levels of a miRNA can regulate protein synthesis in human muscle cells and provide a potential mechanism for muscle wasting in vivo.
Language eng
DOI 10.1152/ajpcell.00172.2020
Indigenous content off
Field of Research 0601 Biochemistry and Cell Biology
0606 Physiology
1116 Medical Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30139860

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in TR Web of Science
Scopus Citation Count Cited 1 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 47 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Fri, 10 Jul 2020, 14:55:59 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.