An obesogenic maternal environment impairs mouse growth patterns, satellite cell activation, and markers of postnatal myogenesis

Mikovic, J, Brightwell, C, Lindsay, Angus, Wen, Y, Kowalski, Grzegorz, Russell, Aaron, Fry, CS and Lamon, Severine 2020, An obesogenic maternal environment impairs mouse growth patterns, satellite cell activation, and markers of postnatal myogenesis, American Journal of Physiology - Endocrinology and Metabolism, vol. 319, no. 6, pp. E1008-E1018, doi: 10.1152/AJPENDO.00398.2020.

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Title An obesogenic maternal environment impairs mouse growth patterns, satellite cell activation, and markers of postnatal myogenesis
Author(s) Mikovic, J
Brightwell, C
Lindsay, AngusORCID iD for Lindsay, Angus orcid.org/0000-0002-5195-1901
Wen, Y
Kowalski, GrzegorzORCID iD for Kowalski, Grzegorz orcid.org/0000-0002-1599-017X
Russell, AaronORCID iD for Russell, Aaron orcid.org/0000-0002-7323-9501
Fry, CS
Lamon, SeverineORCID iD for Lamon, Severine orcid.org/0000-0002-3271-6551
Journal name American Journal of Physiology - Endocrinology and Metabolism
Volume number 319
Issue number 6
Start page E1008
End page E1018
Total pages 11
Publisher American Physiological Society
Place of publication Bethesda, Md.
Publication date 2020-12
ISSN 0193-1849
1522-1555
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Endocrinology & Metabolism
Physiology
fetal programming
microRNA
myogenesis
regeneration
skeletal muscle
SKELETAL-MUSCLE
MACROPHAGES
HEALTH
MECHANISMS
ADIPOSITY
MONOCYTES
SUPPORT
OBESITY
DIET
Summary Skeletal muscle is sensitive to environmental cues that are first present in utero. Maternal overnutrition is a model of impaired muscle development leading to structural and metabolic dysfunction in adult life. In this study, we investigated the effect of an obesogenic maternal environment on growth and postnatal myogenesis in the offspring. Male C57BL/6J mice born to chow- or high-fat-diet-fed mothers were allocated to four different groups at the end of weaning. For the following 10 wk, half of the pups were maintained on the same diet as their mother and half of the pups were switched to the other diet (chow or high-fat). At 12 wk of age, muscle injury was induced using an intramuscular injection of barium chloride. Seven days later, mice were humanely killed and muscle tissue was harvested. A high-fat maternal diet impaired offspring growth patterns and downregulated satellite cell activation and markers of postnatal myogenesis 7 days after injury without altering the number of newly synthetized fibers over the whole 7-day period. Importantly, a healthy postnatal diet could not reverse any of these effects. In addition, we demonstrated that postnatal myogenesis was associated with a diet-independent upregulation of three miRNAs, mmu-miR-31–5p, mmu-miR-136–5p, and mmu-miR-296–5p. Furthermore, in vitro analysis confirmed the role of these miRNAs in myocyte proliferation. Our findings are the first to demonstrate that maternal overnutrition impairs markers of postnatal myogenesis in the offspring and are particularly relevant to today’s society where the incidence of overweight/obesity in women of childbearing age is increasing.
Language eng
DOI 10.1152/AJPENDO.00398.2020
Indigenous content off
Field of Research 06 Biological Sciences
11 Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30143552

Document type: Journal Article
Collections: Faculty of Health
School of Exercise and Nutrition Sciences
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