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Amyloid β induces early changes in the ribosomal machinery, cytoskeletal organization and oxidative phosphorylation in retinal photoreceptor cells

Deng, L, Pushpitha, K, Joseph, C, Gupta, V, Rajput, R, Chitranshi, N, Dheer, Y, Amirkhani, A, Kamath, K, Pascovici, D, Wu, JX, Salekdeh, GH, Haynes, PA, Graham, SL, Gupta, VK and Mirzaei, M 2019, Amyloid β induces early changes in the ribosomal machinery, cytoskeletal organization and oxidative phosphorylation in retinal photoreceptor cells, Frontiers in Molecular Neuroscience, vol. 12, pp. 1-14, doi: 10.3389/fnmol.2019.00024.

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Title Amyloid β induces early changes in the ribosomal machinery, cytoskeletal organization and oxidative phosphorylation in retinal photoreceptor cells
Author(s) Deng, L
Pushpitha, K
Joseph, C
Gupta, V
Rajput, R
Chitranshi, N
Dheer, Y
Amirkhani, A
Kamath, K
Pascovici, D
Wu, JX
Salekdeh, GH
Haynes, PA
Graham, SL
Gupta, VK
Mirzaei, M
Journal name Frontiers in Molecular Neuroscience
Volume number 12
Article ID 24
Start page 1
End page 14
Total pages 14
Publisher Frontiers Media
Place of publication Lausanne, Switzerland
Publication date 2019-02-22
ISSN 1662-5099
1662-5099
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Neurosciences
Neurosciences & Neurology
proteomics
TMT
Alzheimer's disease
photoreceptor
autophagy
amyloid
retina
ALZHEIMERS-DISEASE IMPLICATIONS
MITOCHONDRIAL DYSFUNCTION
MACULAR DEGENERATION
PRECURSOR PROTEIN
TAU
PEPTIDE
DAMAGE
PATHOLOGIES
NEUROTOXICITY
ACCUMULATION
Alzheimer’s disease
Summary Amyloid β (Aβ) accumulation and its aggregation is characteristic molecular feature of the development of Alzheimer’s disease (AD). More recently, Aβ has been suggested to be associated with retinal pathology associated with AD, glaucoma and drusen deposits in age related macular degeneration (AMD). In this study, we investigated the proteins and biochemical networks that are affected by Aβ in the 661 W photoreceptor cells in culture. Time and dose dependent effects of Aβ on the photoreceptor cells were determined utilizing tandem mass tag (TMT) labeling-based quantitative mass-spectrometric approach. Bioinformatic analysis of the data revealed concentration and time dependent effects of the Aβ peptide stimulation on various key biochemical pathways that might be involved in mediating the toxicity effects of the peptide. We identified increased Tau phosphorylation, GSK3β dysregulation and reduced cell viability in cells treated with Aβ in a dose and time dependent manner. This study has delineated for the first-time molecular networks in photoreceptor cells that are impacted early upon Aβ treatment and contrasted the findings with a longer-term treatment effect. Proteins associated with ribosomal machinery homeostasis, mitochondrial function and cytoskeletal organization were affected in the initial stages of Aβ exposure, which may provide key insights into AD effects on the photoreceptors and specific molecular changes induced by Aβ peptide.
Language eng
DOI 10.3389/fnmol.2019.00024
Indigenous content off
Field of Research 1103 Clinical Sciences
1109 Neurosciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2019, Deng, Pushpitha, Joseph, Gupta, Rajput, Chitranshi, Dheer, Amirkhani, Kamath, Pascovici, Wu, Salekdeh, Haynes, Graham, Gupta and Mirzaei
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30144661

Document type: Journal Article
Collections: Faculty of Health
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.