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RNA-Seq analysis of cisplatin and the monofunctional platinum(II) complex, phenanthriplatin, in A549 non-small cell lung cancer and IMR90 lung fibroblast cell lines

Monroe, Jerry D., Moolani, Satya A., Irihamye, Elvin N., Speed, Joshua S., Gibert, Yann and Smith, Michael E. 2020, RNA-Seq analysis of cisplatin and the monofunctional platinum(II) complex, phenanthriplatin, in A549 non-small cell lung cancer and IMR90 lung fibroblast cell lines, Cells, vol. 9, no. 12, pp. 1-17, doi: 10.3390/cells9122637.

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Title RNA-Seq analysis of cisplatin and the monofunctional platinum(II) complex, phenanthriplatin, in A549 non-small cell lung cancer and IMR90 lung fibroblast cell lines
Author(s) Monroe, Jerry D.
Moolani, Satya A.
Irihamye, Elvin N.
Speed, Joshua S.
Gibert, Yann
Smith, Michael E.
Journal name Cells
Volume number 9
Issue number 12
Article ID 2637
Start page 1
End page 17
Total pages 17
Publisher MDPI AG
Place of publication Basel, Switzerland
Publication date 2020-12-08
ISSN 2073-4409
Keyword(s) cancer
cisplatin
monofunctional platinum(II) complex
next generation sequencing
pathway analysis
Summary Phenanthriplatin is a new monofunctional platinum(II) complex that binds only one strand of DNA and acts by blocking gene transcription, but its effect on gene regulation has not been characterized relative to the traditional platinum-based complex, cisplatin. A549 non-small cell lung cancer and IMR90 lung fibroblast cells were treated with cisplatin, phenanthriplatin, or a control and then their RNA transcripts were subjected to next generation sequencing analysis. DESeq2 and CuffDiff2 were used to identify up- and downregulated genes and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases were used to identify pathways and functions. We found that phenanthriplatin may regulate the genes GPRC5a, TFF1, and TNFRSF10D, which act through p53 to control apoptosis, differently or to a greater extent than cisplatin, and that it, unlike cisplatin, could upregulate ATP5MD, a gene which signals through the Wnt/β catenin pathway. Furthermore, phenanthriplatin caused unique or enhanced effects compared to cisplatin on genes regulating the cytoskeleton, cell migration, and proliferation, e.g., AGAP1, DIAPH2, GDF15, and THSD1 (p < 0.05; q < 0.05). Phenanthriplatin may modulate some oncogenes differently than cisplatin potentially leading to improved clinical outcome, but this monofunctional complex should be carefully matched with cancer gene data to be successfully applied in chemotherapy.
Language eng
DOI 10.3390/cells9122637
Indigenous content off
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30146694

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.