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Prevalence and distribution of avian influenza viruses in domestic ducks at the waterfowl-chicken interface in wetlands

Hassan, Mohammad M, Islam, Ariful, Hasan, Rubyath B, Rahman, Md K, Webby, Richard J, Hoque, Md A and El Zowalaty, Mohamed E 2020, Prevalence and distribution of avian influenza viruses in domestic ducks at the waterfowl-chicken interface in wetlands, Pathogens, vol. 9, no. 11, pp. 1-18, doi: 10.3390/pathogens9110953.

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Title Prevalence and distribution of avian influenza viruses in domestic ducks at the waterfowl-chicken interface in wetlands
Author(s) Hassan, Mohammad M
Islam, ArifulORCID iD for Islam, Ariful orcid.org/0000-0002-9210-3351
Hasan, Rubyath B
Rahman, Md K
Webby, Richard J
Hoque, Md A
El Zowalaty, Mohamed E
Journal name Pathogens
Volume number 9
Issue number 11
Article ID 953
Start page 1
End page 18
Total pages 18
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2020-11
ISSN 2076-0817
2076-0817
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Microbiology
avian influenza
prevalence
risk factors
real-time reverse-transcription-polymerase chain reaction
c-ELISA
waterfowl
ducks
interface
wetlands
Bangladesh
Summary Ducks are a natural reservoir of influenza A viruses (IAVs) and can act as a reassortment vessel. Wetlands, such as Hakaluki and Tanguar haor in Bangladesh, have unique ecosystems including domestic duck (Anas platyrhynchos domesticus) rearing, especially household and free-range ducks. A cross-sectional study was, therefore, conducted to explore avian influenza status and its distribution and risk factors in the wetland areas. During the three consecutive winters of 2015–2017, specifically in December of these years, we collected a total of 947 samples including blood, oropharyngeal and cloacal swabs from domestic ducks (such as free-range ducks (n = 312 samples) and household ducks (n = 635 samples) in wetlands. We screened serum samples using a nucleoprotein competitive enzyme-linked immunosorbent assay (c-ELISA) to estimate seroprevalence of IAV antibodies and swab samples by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) to detect IA viral M gene. Eleven M gene positive samples were subjected to sequencing and phylogenetic analysis. Serological and viral prevalence rates of IAVs were 63.8% (95% CI: 60.6–66.8) and 10.7% (8.8–12.8), respectively. Serological and viral RNA prevalence rates were 51.8% (95% CI: 47.2–56.4) and 10.2% (7.6–13.3) in Hakaluki haor, 75.6% (71.5–79.4) and 11.1% (8.5–14.3) in Tanguar haor, 66.3% (62.5–69.9) and 11.2% (8.8–13.9) in household ducks and 58.7% (52.9–64.2) and 9.6% (6.5–13.4) in free-range ducks, respectively. The risk factors identified for higher odds of AI seropositive ducks were location (OR = 2.9, 95% CI: 2.2–3.8, p < 0.001; Tanguar haor vs. Hakaluki haor), duck-rearing system (OR = 1.4, 1.1–1.8, household vs. free-range), farmer’s education status (OR = 1.5, 1.2–2.0, p < 0.05 illiterate vs. literate) and contact type (OR = 3.0, 2.1–4.3, p < 0.001; contact with chicken vs. no contact with chicken). The risk factors identified for higher odds of AI viral RNA positive ducks were farmer’s education status (OR = 1.5, 1.0–2.3, p < 0.05 for illiterate vs literate), contact type (OR = 2.7, 1.7–4.2, p < 0.001; ducks having contact with chicken vs. ducks having contact with waterfowl). The phylogenetic analysis of 11 partial M gene sequences suggested that the M gene sequences detected in free-range duck were very similar to each other and were closely related to the M gene sequences of previously reported highly pathogenic avian influenza (HPAI) and low pathogenic avian influenza (LPAI) subtypes in waterfowl in Bangladesh and Southeast Asian countries. Results of the current study will help provide significant information for future surveillance programs and model IAV infection to predict the spread of the viruses among migratory waterfowl, free-range ducks and domestic poultry in Bangladesh.
Language eng
DOI 10.3390/pathogens9110953
Indigenous content off
Field of Research 1107 Immunology
1108 Medical Microbiology
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30146835

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.