A molecular barcode and online tool to identify and map imported infection with Plasmodium vivax

Trimarsanto, H, Amato, R, Pearson, RD, Sutanto, E, Noviyanti, R, Trianty, L, Marfurt, J, Pava, Z, Echeverry, DF, Lopera-Mesa, TM, Montenegro, LM, Tobón-Castaño, A, Grigg, MJ, Barber, B, William, T, Anstey, NM, Getachew, S, Petros, B, Aseffa, A, Assefa, A, Rahim, AG, Chau, NH, Hien, TT, Alam, MS, Khan, WA, Ley, B, Thriemer, K, Wangchuck, S, Hamedi, Y, Adam, I, Liu, Y, Gao, Q, Sriprawat, K, Ferreira, MU, Barry, Alyssa, Mueller, I, Drury, E, Goncalves, S, Simpson, V, Miotto, O, Miles, A, White, NJ, Nosten, F, Kwiatkowski, DP, Price, RN and Auburn, S 2019, A molecular barcode and online tool to identify and map imported infection with Plasmodium vivax, bioRxiv (Preprints), pp. 1-28, doi: 10.1101/776781.

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Title A molecular barcode and online tool to identify and map imported infection with Plasmodium vivax
Author(s) Trimarsanto, H
Amato, R
Pearson, RD
Sutanto, E
Noviyanti, R
Trianty, L
Marfurt, J
Pava, Z
Echeverry, DF
Lopera-Mesa, TM
Montenegro, LM
Tobón-Castaño, A
Grigg, MJ
Barber, B
William, T
Anstey, NM
Getachew, S
Petros, B
Aseffa, A
Assefa, A
Rahim, AG
Chau, NH
Hien, TT
Alam, MS
Khan, WA
Ley, B
Thriemer, K
Wangchuck, S
Hamedi, Y
Adam, I
Liu, Y
Gao, Q
Sriprawat, K
Ferreira, MU
Barry, AlyssaORCID iD for Barry, Alyssa orcid.org/0000-0002-1189-2310
Mueller, I
Drury, E
Goncalves, S
Simpson, V
Miotto, O
Miles, A
White, NJ
Nosten, F
Kwiatkowski, DP
Price, RN
Auburn, S
Journal name bioRxiv (Preprints)
Start page 1
End page 28
Total pages 28
Publisher bioRxiv (Preprints)
Publication date 2019-09-24
Summary AbstractImported cases present a considerable challenge to the elimination of malaria. Traditionally, patient travel history has been used to identify imported cases, but the long-latency liver stages confound this approach in Plasmodium vivax. Molecular tools to identify and map imported cases offer a more robust approach, that can be combined with drug resistance and other surveillance markers in high-throughput, population-based genotyping frameworks. Using a machine learning approach incorporating hierarchical FST (HFST) and decision tree (DT) analysis applied to 831 P. vivax genomes from 20 countries, we identified a 28-Single Nucleotide Polymorphism (SNP) barcode with high capacity to predict the country of origin. The Matthews correlation coefficient (MCC), which provides a measure of the quality of the classifications, ranging from −1 (total disagreement) to 1 (perfect prediction), exceeded 0.9 in 15 countries in cross-validation evaluations. When combined with an existing 37-SNP P. vivax barcode, the 65-SNP panel exhibits MCC scores exceeding 0.9 in 17 countries with up to 30% missing data. As a secondary objective, several genes were identified with moderate MCC scores (median MCC range from 0.54-0.68), amenable as markers for rapid testing using low-throughput genotyping approaches. A likelihood-based classifier framework was established, that supports analysis of missing data and polyclonal infections. To facilitate investigator-lead analyses, the likelihood framework is provided as a web-based, open-access platform (vivaxGEN-geo) to support the analysis and interpretation of data produced either at the 28-SNP core or full 65-SNP barcode. These tools can be used by malaria control programs to identify the main reservoirs of infection so that resources can be focused to where they are needed most.
Notes In Press
Language eng
DOI 10.1101/776781
Indigenous content off
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30149569

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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