The influence of disinfectant on development and succession patterns of lucilia sericata in regional Victoria and the implications for PMI estimations

Davis, Jaala 2021, The influence of disinfectant on development and succession patterns of lucilia sericata in regional Victoria and the implications for PMI estimations, B. Forensic Science (Hons) thesis, School of Life and Environmental Sciences, Deakin University.

Attached Files
Name Description MIMEType Size Downloads

Title The influence of disinfectant on development and succession patterns of lucilia sericata in regional Victoria and the implications for PMI estimations
Author Davis, Jaala
Institution Deakin University
School School of Life and Environmental Sciences
Faculty Faculty of Science, Engineering and Built Environment
Degree type Honours
Degree name B. Forensic Science (Hons)
Thesis advisor Harvey, MichelleORCID iD for Harvey, Michelle orcid.org/0000-0002-4047-7845
Conlan XavierORCID iD for Conlan Xavier orcid.org/0000-0003-0829-0551
Date submitted 2021-03-26
Keyword(s) forensic entomology
forensic biology
high performance liquid chromatography
Summary Necrophagous fly species are commonly utilised by forensic entomologists to estimate a minimum post-mortem interval (minPMI) of remains. However, previous studies have revealed that concealment of remains via common household substances to prolong the decomposition process, may influence colonisation and succession patterns of forensically important insects and their subsequent development. This study analysed the impact of a common household disinfectant (Pine O Cleen) on the oviposition preferences, developmental rate and survival of Lucilia sericata (Diptera: Calliphoridae) in a controlled laboratory environment. The impact of disinfectant was also analysed to determine the arrival times and succession patterns of primary colonisers in the Geelong region (Victoria: Australia). Further, High Performance Liquid Chromatography (HPLC) analysis was conducted on soil samples taken from underneath remains, to examine the suitability to detect the presence of substances within decomposition fluids that are released into the surrounding soil. Results demonstrated the presence of disinfectant on remains did not significantly influence the pre-appearance interval (PAI) and colonisation of necrophagous insects. However, flies showed oviposition on piglets exposed to disinfectant was limited to within the body’s natural orifices, avoiding the areas where disinfectant was applied. It was found that seasonality had a significant effect on field studies as carcasses decomposing during winter required more accumulated degree hours (ADH) to progress through putrefaction, though extensive scavenging activity of remains may have hindered this result. Disinfectant was shown to have no significant deterrent effects on the attraction and oviposition of primary colonisers, with L. sericata showing no clear trend in oviposition preference between control and disinfectant treatments. Disinfectant was found to not significantly influence the developmental rate, growth and survival of L. sericata larvae. HPLC analysis of soil samples was able to detect the presence of disinfectant, with no substantial change in chemical4composition observed throughout the decomposition process. These results demonstrate that the presence of disinfectant does not significantly influence the reliability of using entomological data from remains to estimate a minPMI, though further studies should be conducted to confirm whether this is consistent throughout different types of disinfectant.
Language eng
Indigenous content off
Field of Research 3199 Other biological sciences
Description of original 75 p.
Copyright notice ©All rights reserved
Persistent URL http://hdl.handle.net/10536/DRO/DU:30150530

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 25 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Fri, 30 Apr 2021, 15:01:27 EST by Bernadette Admin Houghton

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.