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mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report

Venugopal, Abhirami, Gillick-Walker, J, Michalczyk, Agnieszka, Khasraw, Mustafa and Ackland, Margaret 2021, mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report, BMC gastroenterology, vol. 21, no. 1, pp. 1-12, doi: 10.1186/s12876-021-01705-9.

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Title mRNA profiling of a well-differentiated G1 pancreatic NET correlates with immunohistochemistry profile: a case report
Author(s) Venugopal, Abhirami
Gillick-Walker, J
Michalczyk, AgnieszkaORCID iD for Michalczyk, Agnieszka orcid.org/0000-0001-5716-0783
Khasraw, MustafaORCID iD for Khasraw, Mustafa orcid.org/0000-0003-3249-9849
Ackland, MargaretORCID iD for Ackland, Margaret orcid.org/0000-0002-7474-6556
Journal name BMC gastroenterology
Volume number 21
Issue number 1
Article ID 194
Start page 1
End page 12
Total pages 12
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2021
ISSN 1471-230X
1471-230X
Keyword(s) ANTIGENS
Biomarkers
BREAST-CANCER
CARCINOMAS
Gastroenterology & Hepatology
GENE-EXPRESSION
Immunohistochemistry
KI67
KI-67
Life Sciences & Biomedicine
MARKERS
MRNA
NEUROENDOCRINE TUMORS
Neuroendocrine tumours (NET)
PROTEIN EXPRESSION
QRT-PCR
Science & Technology
Summary Abstract Background Neuroendocrine neoplasms (NENs) are a complex group of tumours that occur in many organs. Routinely used IHC markers for NEN diagnosis include CgA, synaptophysin, Ki67 and CD56. These have limitations including lack of correlation to clinical outcomes and their presence in non-tumour tissue. Identification of additional markers and more quantitative analyses of tumour tissue has the potential to contribute to improved clinical outcomes. We used qRT-PCR to profile the expression levels of a panel of markers in tumour and matched non-tumour tissue from a patient with a G1 pancreatic neuroendocrine tumour. Differences in mRNA levels between tumour and non-tumour tissue were compared with IHC analyses of the same sample. Case presentation An elderly man presented with lower abdominal pain for 6 months. Histological analysis identified a low grade, well differentiated pancreatic endocrine neoplasm. Twenty-seven tumour markers for neuroendocrine status, proliferation, stem cell phenotype, angiogenesis, epithelial to mesenchymal transition, cell adhesion, differentiation and tumour suppression were selected from previous studies and mRNA levels of these markers were measured in tumour and adjacent non-tumour tissue sample using qRT-PCR. IHC was carried out on the same tissue to detect the corresponding marker proteins. Of the markers analysed, seven showed higher mRNA levels in tumour relative to non-tumour tissue while thirteen had lower expression in tumour relative to non-tumour tissue. Substantial differences in mRNA levels were a gain of CgA, CD56, β-catenin, CK20, PDX1 and p53 and loss of Ki67, PCAD, CK7, CD31, MENA, ECAD, EPCAM, CDX2 and CK6. Comparison of qRT-PCR data with IHC showed correlation between fifteen markers. Conclusion Our study is unique as it included matched controls that provided a comparative assessment for tumour tissue analysis, whereas many previous studies report tumour data only. Additionally, we utilised qRT-PCR, a relatively quantitative diagnostic tool for differential marker profiling, having the advantage of being reproducible, fast, cheap and accurate. qRT-PCR has the potential to improve the defining of tumour phenotypes and, in combination with IHC may have clinical utility towards improving tumour stratification or distinguishing tumour grades. The results need to be validated with different grades of NENs and related to clinical outcomes.
Language eng
DOI 10.1186/s12876-021-01705-9
Indigenous content off
Field of Research 1103 Clinical Sciences
1117 Public Health and Health Services
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30150617

Document type: Journal Article
Collections: Institute for Frontier Materials
Open Access Collection
GTP Research
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.