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Metabolite profiling of the Indian food spice lichen, Pseudevernia furfuracea combined with optimised extraction methodology to obtain bioactive phenolic compounds

Kalra, Rishu, Conlan, Xavier A, Areche, Carlos, Dilawari, Rahul and Goel, Mayurika 2021, Metabolite profiling of the Indian food spice lichen, Pseudevernia furfuracea combined with optimised extraction methodology to obtain bioactive phenolic compounds, Frontiers in pharmacology, vol. 12, pp. 1-16, doi: 10.3389/fphar.2021.629695.

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Title Metabolite profiling of the Indian food spice lichen, Pseudevernia furfuracea combined with optimised extraction methodology to obtain bioactive phenolic compounds
Author(s) Kalra, Rishu
Conlan, Xavier AORCID iD for Conlan, Xavier A orcid.org/0000-0003-0829-0551
Areche, Carlos
Dilawari, Rahul
Goel, Mayurika
Journal name Frontiers in pharmacology
Volume number 12
Article ID 629695
Start page 1
End page 16
Total pages 16
Publisher Frontiers Media
Place of publication Lausanne, Switzerland
Publication date 2021-05
ISSN 1663-9812
1663-9812
Keyword(s) antioxidants
atraric acid
lichen
Life Sciences & Biomedicine
metabolomics profiling
olivetoric acid
Pharmacology & Pharmacy
Pseudevernia furfuracea
Science & Technology
spatial localization
UHPLC-MS
Summary Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) is a well-known epiphytic lichen commonly used in Indian spice mixtures and food preparations such as curries. This study is an attempt to find the best extraction methodology with respect to extractive yield, total polyphenolic content (TPC), total flavonoid content and antioxidant activities of lichen P. furfuracea. Two phenolic compounds, atraric acid and olivetoric acid were isolated and quantified in their respective extracts with the aid of reverse phase high performance liquid chromatography (RP-HPLC). The highest concentration of both the compounds, atraric acid (4.89 mg/g DW) and olivetoric acid (11.46 mg/g DW) were found in 70% methanol extract. A direct correlation was also observed between the concentrations of these compounds with the free radical scavenging potential of the extracts which might contribute towards the antioxidant potential of the extract. Moreover, scanning electron microscopy and HPLC analysis which was used to study the effect of pre-processing on extraction process highlighted the capacity of a mixer grinder technique for improved separation of surface localized metabolites and enrichment of the fraction. An investigation of the chemical profile of the bioactive extract 70% methanol extract using UHPLC-DAD-MS lead to tentative identification of forty nine compounds. This extract was also assessed towards HEK 293 T cell line for cytotoxicity analysis. Concentration range of 0.156 to 100 µg/ml of PF70M extract exhibited no significant cell death as compared to control. Further, the active extract showed protective effect against hydroxyl radical’s destructive effects on DNA when assessed using DNA nicking assay. Based upon this, it can be concluded that optimization of extraction solvent, sample pre-proceesing and extraction techniques can be useful in extraction of specific antioxidant metabolites.
Language eng
DOI 10.3389/fphar.2021.629695
Indigenous content off
Field of Research 1115 Pharmacology and Pharmaceutical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30152206

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.