Openly accessible

Characterization of microglial transcriptomes in the brain and spinal cord of mice in early and late experimental autoimmune encephalomyelitis using a RiboTag strategy

Acharjee, S, Gordon, PMK, Lee, BH, Read, Justin, Workentine, ML, Sharkey, KA and Pittman, QJ 2021, Characterization of microglial transcriptomes in the brain and spinal cord of mice in early and late experimental autoimmune encephalomyelitis using a RiboTag strategy, Scientific Reports, vol. 11, pp. 1-13, doi: 10.1038/s41598-021-93590-1.

Attached Files
Name Description MIMEType Size Downloads

Title Characterization of microglial transcriptomes in the brain and spinal cord of mice in early and late experimental autoimmune encephalomyelitis using a RiboTag strategy
Author(s) Acharjee, S
Gordon, PMK
Lee, BH
Read, JustinORCID iD for Read, Justin orcid.org/0000-0003-3790-0873
Workentine, ML
Sharkey, KA
Pittman, QJ
Journal name Scientific Reports
Volume number 11
Article ID 14319
Start page 1
End page 13
Total pages 13
Publisher Springer
Place of publication Berlin, Germany
Publication date 2021
ISSN 2045-2322
2045-2322
Keyword(s) CELLS
CNS
COMPLEMENT
DISEASE
EXPRESSION
MESSENGER-RNA
MONOCYTES
MOUSE MODEL
Multidisciplinary Sciences
MULTIPLE-SCLEROSIS
PROGRESSION
Science & Technology
Science & Technology - Other Topics
Summary AbstractMicroglia play an important role in the pathogenesis of multiple sclerosis and the mouse model of MS, experimental autoimmune encephalomyelitis (EAE). To more fully understand the role of microglia in EAE we characterized microglial transcriptomes before the onset of motor symptoms (pre-onset) and during symptomatic EAE. We compared the transcriptome in brain, where behavioral changes are initiated, and spinal cord, where damage is revealed as motor and sensory deficits. We used a RiboTag strategy to characterize ribosome-bound mRNA only in microglia without incurring possible transcriptional changes after cell isolation. Brain and spinal cord samples clustered separately at both stages of EAE, indicating regional heterogeneity. Differences in gene expression were observed in the brain and spinal cord of pre-onset and symptomatic animals with most profound effects in the spinal cord of symptomatic animals. Canonical pathway analysis revealed changes in neuroinflammatory pathways, immune functions and enhanced cell division in both pre-onset and symptomatic brain and spinal cord. We also observed a continuum of many pathways at pre-onset stage that continue into the symptomatic stage of EAE. Our results provide additional evidence of regional and temporal heterogeneity in microglial gene expression patterns that may help in understanding mechanisms underlying various symptomology in MS.
Language eng
DOI 10.1038/s41598-021-93590-1
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30153515

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 1 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 14 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Tue, 13 Jul 2021, 09:50:36 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.