Openly accessible

PAX2⁺ Mesenchymal Origin of Gonadal Supporting Cells Is Conserved in Birds

Estermann, MA, Mariette, Mylene, Moreau, JLM, Combes, AN and Smith, CA 2021, PAX2⁺ Mesenchymal Origin of Gonadal Supporting Cells Is Conserved in Birds, Frontiers in Cell and Developmental Biology, vol. 9, pp. 1-13, doi: 10.3389/fcell.2021.735203.

Attached Files
Name Description MIMEType Size Downloads

Title PAX2⁺ Mesenchymal Origin of Gonadal Supporting Cells Is Conserved in Birds
Author(s) Estermann, MA
Mariette, MyleneORCID iD for Mariette, Mylene orcid.org/0000-0003-0567-4111
Moreau, JLM
Combes, AN
Smith, CA
Journal name Frontiers in Cell and Developmental Biology
Volume number 9
Article ID 735203
Start page 1
End page 13
Total pages 13
Publisher Frontiers
Place of publication Lausanne, Switzerland
Publication date 2021-08
ISSN 2296-634X
2296-634X
Keyword(s) Cell Biology
DETERMINING GENE
Developmental Biology
DMRT1
embryonic gonad
Evo-Devo
FATE
gonadal sex differentiation
Life Sciences & Biomedicine
LINEAGE SPECIFICATION
MALE PATHWAY
MALE SEX DETERMINATION
MOUSE
PAX2
Science & Technology
sex determination
SOMATIC-CELLS
SUPPRESSES MEIOSIS
ZEBRA FINCH
Summary During embryonic gonadal development, the supporting cell lineage is the first cell type to differentiate, giving rise to Sertoli cells in the testis and pre-granulosa cells in the ovary. These cells are thought to direct other gonadal cell lineages down the testis or ovarian pathways, including the germline. Recent research has shown that, in contrast to mouse, chicken gonadal supporting cells derive from a PAX2/OSR1/DMRT1/WNT4 positive mesenchymal cell population. These cells colonize the undifferentiated genital ridge during early gonadogenesis, around the time that germ cells migrate into the gonad. During the process of somatic gonadal sex differentiation, PAX2 expression is down-regulated in embryonic chicken gonads just prior to up-regulation of testis- and ovary-specific markers and prior to germ cell differentiation. Most research on avian gonadal development has focused on the chicken model, and related species from the Galloanserae clade. There is a lack of knowledge on gonadal sex differentiation in other avian lineages. Comparative analysis in birds is required to fully understand the mechanisms of avian sex determination and gonadal differentiation. Here we report the first comparative molecular characterization of gonadal supporting cell differentiation in birds from each of the three main clades, Galloanserae (chicken and quail), Neoaves (zebra finch) and Palaeognathe (emu). Our analysis reveals conservation of PAX2+ expression and a mesenchymal origin of supporting cells in each clade. Moreover, down-regulation of PAX2 expression precisely defines the onset of gonadal sex differentiation in each species. Altogether, these results indicate that gonadal morphogenesis is conserved among the major bird clades
Language eng
DOI 10.3389/fcell.2021.735203
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30156013

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 7 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Fri, 01 Oct 2021, 15:34:23 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.