•  Home
  • Library
  • DRO home
Submit research Contact DRO

DRO

Openly accessible

Diverse Bacterial Resistance Genes Detected in Fecal Samples From Clinically Healthy Women and Infants in Australia—A Descriptive Pilot Study

Guernier, V, Chamings, Anthony, Collier, F and Alexandersen, Soren 2021, Diverse Bacterial Resistance Genes Detected in Fecal Samples From Clinically Healthy Women and Infants in Australia—A Descriptive Pilot Study, Frontiers in Microbiology, vol. 12, pp. 1-12, doi: 10.3389/fmicb.2021.596984.

Attached Files
Name Description MIMEType Size Downloads

Title Diverse Bacterial Resistance Genes Detected in Fecal Samples From Clinically Healthy Women and Infants in Australia—A Descriptive Pilot Study
Author(s) Guernier, V
Chamings, AnthonyORCID iD for Chamings, Anthony orcid.org/0000-0002-7762-4757
Collier, F
Alexandersen, SorenORCID iD for Alexandersen, Soren orcid.org/0000-0002-5039-3178
Journal name Frontiers in Microbiology
Volume number 12
Article ID 596984
Start page 1
End page 12
Total pages 12
Publisher Frontiers
Place of publication Lausanne, Switzerland
Publication date 2021-09
ISSN 1664-302X
1664-302X
Keyword(s) AMR
antimicrobial resistance
CATTLE
IDENTIFICATION
Life Sciences & Biomedicine
metagenomics
Microbiology
NGS
resistance genes
resistome
Science & Technology
Summary The gut microbiota is an immense reservoir of antimicrobial resistance genes (ARGs), the so-called “resistome.” In Australia, where antibiotic use is high and resistance rates in some common pathogens are increasing, very little is known about the human resistome. To assess the presence and diversity of ARGs in the gut of Australians from south-eastern Victoria, we investigated fecal samples from clinically healthy infants and pregnant women using non-targeted (shotgun metagenomics sequencing or SMS) and targeted sequencing (two Ion AmpliseqTM panels). All methods detected ARGs in all samples, with the detection overall of 64 unique genes conferring resistance to 12 classes of antibiotics. Predominant ARGs belonged to three classes of antibiotics that are the most frequently prescribed in Australia: tetracycline, β-lactams and MLSB (macrolide, lincosamide, streptogramin B). The three bacterial Orders commonly identified as carrying ARGs were Clostridiales, Bacteroidales, and Enterobacteriales. Our preliminary results indicate that ARGs are ubiquitously present and diverse among the gut microbiota of clinically healthy humans from south-eastern Victoria, Australia. The observed resistance pattern partly overlaps with antimicrobial usage in human medicine in Australia, but ARGs to tetracycline are more common than could be expected. Our current sample is small and limited to south-eastern Victoria, and more data on healthy individuals will be needed to better depict resistance patterns at the population level, which could guide population and/or environmental monitoring and surveillance of antibiotic resistance on various spatio-temporal scales in Australia. For future studies, we recommend using the Ion AmpliseqTM Antimicrobial Resistance Research panel, which is sensitive and user-friendly, or combining several methods to increase the detected diversity
Language eng
DOI 10.3389/fmicb.2021.596984
Field of Research 0502 Environmental Science and Management
0503 Soil Sciences
0605 Microbiology
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30156797

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
Related Links
Link Description
Link to full-text (open access)  
Connect to Elements publication management system
Go to link with your DU access privileges
 
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus Google Scholar Search Google Scholar
Access Statistics: 73 Abstract Views, 1 File Downloads  -  Detailed Statistics
Created: Sun, 10 Oct 2021, 10:00:07 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.