Lef1 enhances the progression of colonic adenocarcinoma via remodeling the cell motility associated structures Xiao, L, Zhang, C, Li, X, Jia, C, Chen, L, Yuan, Y, Gao, Q, Lu, Z, Feng, Y, Zhao, R, Zhao, X, Cheng, S, Shu, Z, Xu, J, Duan, Wei, Nie, G and Hou, Y 2021, Lef1 enhances the progression of colonic adenocarcinoma via remodeling the cell motility associated structures, International Journal of Molecular Sciences, vol. 22, no. 19, pp. 1-14, doi: 10.3390/ijms221910870. Attached Files Name Description MIMEType Size Downloads Title Lef1 enhances the progression of colonic adenocarcinoma via remodeling the cell motility associated structures Author(s) Xiao, L Zhang, C Li, X Jia, C Chen, L Yuan, Y Gao, Q Lu, Z Feng, Y Zhao, R Zhao, X Cheng, S Shu, Z Xu, J Duan, Wei orcid.org/0000-0001-5782-9184 Nie, G Hou, Y Journal name International Journal of Molecular Sciences Volume number 22 Issue number 19 Article ID 10870 Start page 1 End page 14 Total pages 14 Publisher MDPI Place of publication Basel, Switzerland Publication date 2021-10 ISSN 1661-6596 1422-0067 Keyword(s) Biochemistry & Molecular Biology CANCER cancer malignancy CATENIN cell microstructures Chemistry Chemistry, Multidisciplinary colonic cancer CYTOSKELETON EMT EXPRESSION INVASION LEF1 Life Sciences & Biomedicine MIGRATION NOTCH Physical Sciences Science & Technology Summary Lymphoid enhancer-binding factor 1 (LEF1) is a key transcription factor mediating the Wnt signaling pathway. LEF1 is a regulator that is closely associated with tumor malignancy and is usually upregulated in cancers, including colonic adenocarcinoma. The underlying molecular mechanisms of LEF1 regulation for colonic adenocarcinoma progression remain unknown. To explore it, the LEF1 expression in caco2 cells was inhibited using an shRNA approach. The results showed that downregulation of LEF1 inhibited the malignancy and motility associated microstructures, such as polymerization of F-actin, β-tubulin, and Lamin B1 in caco2 cells. LEF1 inhibition suppressed the expression of epithelial/endothelial-mesenchymal transition (EMT) relevant genes. Overall, the current results demonstrated that LEF1 plays a pivotal role in maintaining the malignancy of colonic adenocarcinoma by remodeling motility correlated microstructures and suppressing the expression of EMT-relevant genes. Our study provided evidence of the roles LEF1 played in colonic adenocarcinoma progression, and suggest LEF1 as a potential target for colonic adenocarcinoma therapy Language eng DOI 10.3390/ijms221910870 Field of Research 0399 Other Chemical Sciences 0604 Genetics 0699 Other Biological Sciences HERDC Research category C1 Refereed article in a scholarly journal Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30157043 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Link to full-text (open access)   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Fri, 15 Oct 2021, 08:00:35 EST Fri, 15 Oct 2021, 15:27:23 EST Mon, 18 Oct 2021, 14:28:13 EST Sat, 23 Oct 2021, 01:44:26 EST Fri, 12 Nov 2021, 05:12:48 EST Sat, 13 Nov 2021, 01:31:54 EST Thu, 16 Dec 2021, 09:59:32 EST Thu, 16 Dec 2021, 13:32:26 EST Thu, 16 Dec 2021, 13:34:03 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 47 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Fri, 15 Oct 2021, 08:00:35 EST