Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat Zhou, J, Li, L, Cui, D, Xie, X, Yang, Wenrong and Yan, F 2021, Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat, BioMedical Engineering Online, vol. 20, pp. 1-12, doi: 10.1186/s12938-021-00954-9. Attached Files Name Description MIMEType Size Downloads Title Effects of gold nanoparticles combined with human β-defensin 3 on the alveolar bone loss of periodontitis in rat Author(s) Zhou, J Li, L Cui, D Xie, X Yang, Wenrong orcid.org/0000-0001-8815-1951 Yan, F Journal name BioMedical Engineering Online Volume number 20 Start page 1 End page 12 Total pages 12 Publisher BioMed Central Place of publication London, Eng. Publication date 2021 ISSN 1475-925X Keyword(s) Gold nanoparticles Osteogenesis Periodontitis hBD3 Summary Background: Nanomaterials of biomedicine and tissue engineering have been proposed for the treatment of periodontitis in recent years. This study aimed to investigate the effects of gold nanoparticles (AuNPs) combined with human β-defensin 3 (hBD3) on the repair of the alveolar bones of experimental periodontitis in rats. Methods: A model of experimental periodontitis was established by ligation of the maxillary second molars with silk thread in rats, which were treated with or without AuNPs combined with hBD3. Micro‐computerized tomography (micro-CT) scanning, enzyme-linked immunosorbent assay, and histological and immunohistochemical staining, including alkaline phosphatase (ALP), osteoprotegerin (OPG), tartrate-resistant acid phosphatase (TRAP), and receptor activator of NF-κB ligand (RANKL), were used to analyze the samples. Results: Micro-CT demonstrated that the alveolar bone resorption was significantly reduced after the treatment with AuNPs combined with hBD3. Levels of TNF-α and IL-6 were decreased markedly compared with the ligation group. H&E and Masson staining showed that AuNPs combined with hBD3 group had less inflammatory cell infiltration, collagen fibrosis and fracture, but higher calcification in the new bone tissue. Moreover, the administration of AuNPs combined with hBD3 increased the expression levels of ALP and OPG (related to bone formation) while decreasing the expression levels of TRAP and RANKL (related to bone resorption) expression. Conclusions: AuNPs combined with hBD3 had a protective effect on the progression of experimental periodontitis in rats and played a certain role in suppressing osteoclastogenesis and alleviating the inflammatory destruction of periodontitis along with the promotion of bone repair. Language eng DOI 10.1186/s12938-021-00954-9 Field of Research 0903 Biomedical Engineering HERDC Research category C1 Refereed article in a scholarly journal Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30160194 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Open Access Collection Related Links Link Description Link to full-text (open access)   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Fri, 17 Dec 2021, 14:02:30 EST Fri, 31 Dec 2021, 14:45:48 EST Fri, 31 Dec 2021, 14:55:43 EST Fri, 31 Dec 2021, 16:25:33 EST Thu, 03 Feb 2022, 10:45:38 EST Thu, 03 Feb 2022, 13:52:12 EST Thu, 03 Feb 2022, 13:55:36 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 31 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Fri, 17 Dec 2021, 14:02:30 EST