Optimization of aqueous enzyme-assisted extraction of rosmarinic acid from rosemary (Rosmarinus officinalis L.) leaves and the antioxidant activity of the extract
Nguyen, Hoang Chinh, Nguyen, HNT, Huang, MY, Lin, KH, Pham, DC, Tran, YB and Su, CH 2021, Optimization of aqueous enzyme-assisted extraction of rosmarinic acid from rosemary (Rosmarinus officinalis L.) leaves and the antioxidant activity of the extract, Journal of Food Processing and Preservation, vol. 45, no. 3, doi: 10.1111/jfpp.15221.
Attached Files
Name
Description
MIMEType
Size
Downloads
Title
Optimization of aqueous enzyme-assisted extraction of rosmarinic acid from rosemary (Rosmarinus officinalis L.) leaves and the antioxidant activity of the extract
Rosemary (Rosmarinus officinalis L.) is a potential source of rosmarinic acid (RA). This study aimed to develop a novel aqueous enzymatic approach for extracting RA from rosemary leaves and evaluate the antioxidant activity of the RA-rich extract. The ability of several enzymes to release RA from plant tissues was investigated. Among the examined enzymes, cellulase A exhibited the highest extraction efficiency. Subsequently, aqueous cellulase A-assisted extraction was optimized. The optimal extraction conditions were as follows: an extraction time of 4.63 hr, a water-to-sample ratio of 28.69 ml/g, an enzyme concentration of 2.56%, and a temperature of 36.6°C, with the maximum RA content of 13.97 mg/g. The RA-rich extract exhibited potent antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl radical with an IC50 value of 532.01 μg/ml. Aqueous cellulase-assisted extraction is a green approach for RA extraction from rosemary leaves. Furthermore, the rosemary leaf extract is a promising antioxidant agent for future use. Practical applications: RA is a potent antioxidant and is widely applied in the food and pharmaceutical industries. This study developed a new method involving the use of enzyme for ecofriendly extracting RA from rosemary leaves. The results indicated that the RA-rich extract is a potential antioxidant agent for future nutraceutical and pharmaceutical applications and the aqueous cellulase-assisted extraction is a practical and green process for RA extraction from rosemary leaves.
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.
Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO.
If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.
Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.
Cited 
Cited 