The NGS Magic Pudding: A Nanopore-Led Long-Read Genome Assembly for the Commercial Australian Freshwater Crayfish, Cherax destructor Austin, CM, Croft, Larry, Grandjean, F and Gan, HM 2022, The NGS Magic Pudding: A Nanopore-Led Long-Read Genome Assembly for the Commercial Australian Freshwater Crayfish, Cherax destructor, Frontiers in Genetics, vol. 12, pp. 1-8, doi: 10.3389/fgene.2021.695763. Attached Files Name Description MIMEType Size Downloads Title The NGS Magic Pudding: A Nanopore-Led Long-Read Genome Assembly for the Commercial Australian Freshwater Crayfish, Cherax destructor Author(s) Austin, CM Croft, Larry orcid.org/0000-0002-4588-6170 Grandjean, F Gan, HM Journal name Frontiers in Genetics Volume number 12 Article ID 695763 Start page 1 End page 8 Total pages 8 Publisher Frontiers Media SA Place of publication Lausanne, Switzerland Publication date 2022-01-19 ISSN 1664-8021 Keyword(s) genome annotation nanopore cellulase aquacultrure decapoda Parastacidae Summary Cherax destructor, the yabby, is an iconic Australian freshwater crayfish species, which, similar to other major invertebrate groups, is grossly under-represented in genomic databases. The yabby is also the principal commercial freshwater crustacean species in Australia subject to explotation via inland fisheries and aquaculture. To address the genomics knowledge gap for this species and explore cost effective and efficient methods for genome assembly, we generated 106.8 gb of Nanopore reads and performed a long-read only assembly of the Cherax destructor genome. On a mini-server configured with an ultra-fast swap space, the de novo assembly took 131 h (∼5.5 days). Genome polishing with 126.3 gb of PCR-Free Illumina reads generated an assembled genome size of 3.3 gb (74.6% BUSCO completeness) with a contig N50 of 80,900 bp, making it the most contiguous for freshwater crayfish genome assemblies. We found an unusually large number of cellulase genes within the yabby genome which is relevant to understanding the nutritional biology, commercial feed development, and ecological role of this species and crayfish more generally. These resources will be useful for genomic research on freshwater crayfish and our methods for rapid and super-efficient genome assembly will have wide application. Language eng DOI 10.3389/fgene.2021.695763 Indigenous content off Field of Research 0604 Genetics 1103 Clinical Sciences 1801 Law HERDC Research category C1 Refereed article in a scholarly journal Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30162443 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Open Access Collection Related Links Link Description Link to full-text (open access)   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Mon, 14 Feb 2022, 07:16:38 EST Mon, 14 Feb 2022, 15:33:14 EST Mon, 14 Feb 2022, 15:41:41 EST Wed, 16 Feb 2022, 16:09:49 EST Tue, 08 Mar 2022, 09:25:47 EST Tue, 08 Mar 2022, 14:38:49 EST Tue, 08 Mar 2022, 14:44:46 EST Tue, 08 Mar 2022, 14:54:22 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 11 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 14 Feb 2022, 07:16:38 EST