A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling Muñoz, SS, Engel, M, Balez, R, Do-Ha, D, Cabral-da-Silva, MC, Hernández, D, Berg, T, Fifita, JA, Grima, N, Yang, S, Blair, IP, Nicholson, G, Cook, AL, Hewitt, AW, Pébay, A and Ooi, L 2020, A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling, Cells, vol. 9, no. 9, pp. 1-17, doi: 10.3390/cells9092018. Attached Files Name Description MIMEType Size Downloads Title A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling Author(s) Muñoz, SS Engel, M Balez, R Do-Ha, D Cabral-da-Silva, MC Hernández, D Berg, T Fifita, JA Grima, N Yang, S Blair, IP Nicholson, G Cook, AL Hewitt, AW Pébay, A Ooi, L Journal name Cells Volume number 9 Issue number 9 Article ID 2018 Start page 1 End page 17 Total pages 17 Publisher MDPI Place of publication Basel, Switzerland Publication date 2020 ISSN 2073-4409 Keyword(s) Science & Technology Life Sciences & Biomedicine Cell Biology induced pluripotent stem cells disease modelling neuronal differentiation cholinergic neurons Alzheimer's disease frontotemporal dementia APOLIPOPROTEIN-E TYPE-4 ALLELE 5-FLUORO-2'-DEOXYURIDINE PROLIFERATION EXPRESSION BIOLOGY EMX1 Alzheimer’s disease Summary The study of neurodegenerative diseases using pluripotent stem cells requires new methods to assess neurodevelopment and neurodegeneration of specific neuronal subtypes. The cholinergic system, characterized by its use of the neurotransmitter acetylcholine, is one of the first to degenerate in Alzheimer's disease and is also affected in frontotemporal dementia. We developed a differentiation protocol to generate basal forebrain-like cholinergic neurons (BFCNs) from induced pluripotent stem cells (iPSCs) aided by the use of small molecule inhibitors and growth factors. Ten iPSC lines were successfully differentiated into BFCNs using this protocol. The neuronal cultures were characterised through RNA and protein expression, and functional analysis of neurons was confirmed by whole-cell patch clamp. We have developed a reliable protocol using only small molecule inhibitors and growth factors, while avoiding transfection or cell sorting methods, to achieve a BFCN culture that expresses the characteristic markers of cholinergic neurons. Language eng DOI 10.3390/cells9092018 HERDC Research category C1 Refereed article in a scholarly journal Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30164046 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Link to full-text (open access)   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 10 Mar 2022, 07:06:38 EST Thu, 10 Mar 2022, 16:01:44 EST Thu, 10 Mar 2022, 16:04:11 EST Sat, 19 Mar 2022, 01:33:09 EST Sat, 19 Mar 2022, 01:48:26 EST Wed, 06 Apr 2022, 09:00:50 EST Wed, 20 Apr 2022, 17:01:56 EST Wed, 20 Apr 2022, 17:14:05 EST Wed, 20 Apr 2022, 17:19:02 EST Filtered Full Citation counts:   Cited 7 times in TR Web of Science Cited 9 times in Scopus Search Google Scholar Access Statistics: 7 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Thu, 10 Mar 2022, 07:06:38 EST