The antioxidant potential of black cumin (Nigella sativa l.) extracts through different extraction methods Iqbal, MJ, Butt, MS, Sohail, M and Suleria, Hafiz 2019, The antioxidant potential of black cumin (Nigella sativa l.) extracts through different extraction methods, Current Bioactive Compounds, vol. 15, no. 6, pp. 623-630, doi: 10.2174/1573407214666180821124454. Title The antioxidant potential of black cumin (Nigella sativa l.) extracts through different extraction methods Author(s) Iqbal, MJ Butt, MS Sohail, M Suleria, Hafiz orcid.org/0000-0001-7045-935X Journal name Current Bioactive Compounds Volume number 15 Issue number 6 Start page 623 End page 630 Total pages 8 Publisher Bentham Science Place of publication Beijing, China Publication date 2019 ISSN 1573-4072 1875-6646 Keyword(s) Black cumin supercritical antioxidant potential HPLC thymoquinone Summary Background: Herbal foods possess significant amounts of essential bioactive nutrients and offer clinical and therapeutic benefits. The aim of present research was to compare the antioxidant potential of black cumin extracts obtained using different extraction modes. Materials and Methods: Three solvents (methanol, acetone and hexane) were employed at different time intervals and ratios. However, Supercritical Fluid Extracts (SFE) were obtained at varying pressures (5500, 6500 and 7500 psi). Results: Outcomes revealed that total phenolics were maximum in methanolic extract (790.79±31.67 mg GAE/100 g) at 50 min. Similarly, DPPH and FRAP activity of methanolic extract was also better than other extracts as 80.99±4.02% and 27.44±0.88 mg TE/g, respectively. HPLC quantification exhibited that highest recovery of major bioactive component (thymoquinone) was obtained via supercritical extraction (12.38 mg/g) at 7500 psi. Conclusions: It is concluded that Pakistani variety of black cumin possess appreciable antioxidant activity which directly depends upon the extraction conditions. Among conventional solvents, methanol was evidenced to be more efficient. However, supercritical fluid extracts have proved a reliable mean for the extraction of thymoquinone. Language eng DOI 10.2174/1573407214666180821124454 Field of Research 1115 Pharmacology and Pharmaceutical Sciences HERDC Research category C1.1 Refereed article in a scholarly journal Persistent URL http://hdl.handle.net/10536/DRO/DU:30171301 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Related Links Link Description Connect to published version Go to link with your DU access privileges   Connect to Elements publication management system Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Tue, 31 May 2022, 09:02:57 EST Tue, 31 May 2022, 15:56:58 EST Tue, 31 May 2022, 16:10:20 EST Mon, 25 Jul 2022, 12:42:54 EST Tue, 26 Jul 2022, 16:06:18 EST Wed, 27 Jul 2022, 13:51:39 EST Wed, 27 Jul 2022, 13:55:07 EST Thu, 28 Jul 2022, 14:04:13 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 4 times in Scopus Search Google Scholar Access Statistics: 6 Abstract Views  -  Detailed Statistics Created: Tue, 31 May 2022, 09:02:57 EST