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Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption

Tuck, B, Watkin, E, Somers, Anthony, Forsyth, Maria and Machuca, LL 2022, Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption, Microorganisms, vol. 10, no. 6, pp. 1-13, doi: 10.3390/microorganisms10061227.

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Title Enhancing Biocide Efficacy: Targeting Extracellular DNA for Marine Biofilm Disruption
Author(s) Tuck, B
Watkin, E
Somers, AnthonyORCID iD for Somers, Anthony orcid.org/0000-0002-0220-2904
Forsyth, MariaORCID iD for Forsyth, Maria orcid.org/0000-0002-4273-8105
Machuca, LL
Journal name Microorganisms
Volume number 10
Issue number 6
Start page 1
End page 13
Total pages 13
Publisher MDPI AG
Place of publication Basel, Switzerland
Publication date 2022
ISSN 2076-2607
2076-2607
Keyword(s) biocide enhancement
biofilm
corrosion inhibitor
EPS
extracellular DNA
extracellular polymeric substances
microbiologically influenced corrosion
Summary Biofilm formation is a global health, safety and economic concern. The extracellular composition of deleterious multispecies biofilms remains uncanvassed, leading to an absence of targeted biofilm mitigation strategies. Besides economic incentives, drive also exists from industry and research to develop and apply environmentally sustainable chemical treatments (biocides); especially in engineered systems associated with the marine environment. Recently, extracellular DNA (eDNA) was implicated as a critical structural polymer in marine biofilms. Additionally, an environmentally sustainable, multi-functional biocide was also introduced to manage corrosion and biofilm formation. To anticipate biofilm tolerance acquisition to chemical treatments and reduce biocide application quantities, the present research investigated eDNA as a target for biofilm dispersal and potential enhancement of biocide function. Results indicate that mature biofilm viability can be reduced by two-fold using reduced concentrations of the biocide alone (1 mM instead of the recommended 10 mM). Importantly, through the incorporation of an eDNA degradation stage, biocide function could be enhanced by a further ~90% (one further log reduction in viability). Biofilm architecture analysis post-treatment revealed that endonuclease targeting of the matrix allowed greater biocide penetration, leading to the observed viability reduction. Biofilm matrix eDNA is a promising target for biofilm dispersal and antimicrobial enhancement in clinical and engineered systems.
Language eng
DOI 10.3390/microorganisms10061227
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30174489

Document type: Journal Article
Collections: Institute for Frontier Materials
Open Access Collection
GTP Research
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.